A high molecular weight form of terminal deoxynucleotidyl transferase

Abstract

CALF thymus terminal deoxynucleotidyl transferase (TdT) (EC 2.7.7.31) is a low molecular weight protein containing two peptides of MWs 24,000 and 8,000 in a native structure estimated to have a molecular weight of 32,000 (ref. 1). Use of antibody directed against homogeneous bovine transferase2 for immunofluorescent cytology showed the normal thymus localisation to be cytoplasmic and nuclear, whereas bone marrow localisation is only nuclear3. Several human lymphoblastoid cell lines that contain terminal transferase activity also exhibit the nuclear location by immunofluorescence4. As cultured lymphoblastoid cell lines are readily labelled with radioactive amino acids we surveyed the peptides present in the immuno-precipitated transferase from these lines by fluorography of SDS gels. The results presented here show that a single 58,000-MW peptide is precipitated by monospecific rabbit antibody against bovine transferase. No peptides as small as those peptides present in calf thymus transferase were detected.

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References

  1. 1

    Chang, L. M. S. & Bollum, F. J. J. biol. Chem. 246, 909–916 (1971).

    CAS  PubMed  Google Scholar 

  2. 2

    Bollum, F. J. Proc. natn. Acad. Sci. U.S.A. 72, 4119–4122 (1975).

    ADS  CAS  Article  Google Scholar 

  3. 3

    Gregoire, K. E., Goldschneider, I., Barton, R. W. & Bollum, F. J. Proc. natn. Acad. Sci. U.S.A. 74, 3993–3996 (1977).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Bollum, F. J. Adv. Enzym. 47, 347–374 (1978).

    CAS  Google Scholar 

  5. 5

    Huang, C. C., Hou, Y., Woods, L. K., Moore, G. E. & Minowada, J. J. natn. Cancer Inst. 53, 655–660 (1974).

    CAS  Article  Google Scholar 

  6. 6

    Foley, G. E. et al. Cancer 18, 522–529 (1965).

    CAS  Article  Google Scholar 

  7. 7

    Laemmli, U. K. Nature 277, 680–685 (1970).

    ADS  Article  Google Scholar 

  8. 8

    Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    CAS  Article  Google Scholar 

  9. 9

    Pazmiño, N. H., McEwan, R. N. & Ihle, J. N. J. Immun. 119, 494–499 (1977).

    PubMed  Google Scholar 

  10. 10

    McCaffery, R., Harrison, T. A., Parkman, R. & Baltimore, D. New Engl. J. Med. 292, 775–780 (1975).

    Article  Google Scholar 

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BOLLUM, F., BROWN, M. A high molecular weight form of terminal deoxynucleotidyl transferase. Nature 278, 191–192 (1979). https://doi.org/10.1038/278191a0

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