Abstract
THE mechanism of replication of the 5′ ends of linear DNA chromosomes remains unclear1,2, as the known DNA polymerases, which act in the 5′ to 3′ direction, require a free 3′-OH as a primer for chain elongation. If a short ribonucleotide sequence primed DNA synthesis, its subsequent removal would leave no primer for gap fill synthesis at the 5′ end. Most linear bacteriophage DNA molecules therefore have terminal repeats or single stranded complementary ends, which allow formation of circular or concatemeric replication intermediates1. Several interesting models have been proposed to explain replication of the 5′ ends of eukaryote chromosomes and linear DNA molecules which cannot circularise. Cavalier-Smith3 proposed that chromosome ends can form self-complementary hairpin loops, allowing the parental 3′ end to act as a primer for DNA polymerase, followed by endonuclease action and repair synthesis. This model has been modified by Bateman4 and Tattersall and Ward5 have extended it to propose a rolling hairpin model for the replication of parvovirus and linear chromosomal DNA. There is now considerable evidence for hairpin priming of replication of the DNA of minute virus of mice (MVM) and adeno-associated virus (AAV)5–7, and hairpin priming may well be a general mechanism for replication of linear DNA molecules that are unable to circularise or form concatemers3,5,7. Roberts8 and Wu et al.9 have proposed a hairpin-primed model for replication of adenovirus DNA, which is summarised in Fig. 1. We present evidence here which is not consistent with a hairpin-primed model for replication of adenovirus DNA.
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STILLMAN, B., BELLETT, A. & ROBINSON, A. Replication of linear adenovirus DNA is not hairpin-primed. Nature 269, 723–725 (1977). https://doi.org/10.1038/269723a0
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DOI: https://doi.org/10.1038/269723a0
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