In vitro synthesis of infectious DNA of murine leukaemia virus

Abstract

DNA synthesised in vitro by purified virions of murine leukaemia virus is infectious. Neither RNA nor protein is required for infectivity. Transfection with reverse transcriptase product shows a single-hit dose response and results in the production of complete, infectious virus.

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References

  1. 1

    Baltimore, D. Nature 226, 1209–1211 (1970).

  2. 2

    Temin, H. M. & Mizutani, S. Nature 226, 1211–1213 (1970).

  3. 3

    Boettiger, D. & Temin, H. M. Nature 228, 622–624 (1970).

  4. 4

    Balduzzi, P. & Morgan, H. R. J. Virol. 5, 470–477 (1970).

  5. 5

    Junghans, R. P., Duesberg, P. H. & Knight, C. A. Proc. natn. Acad. Sci. U.S.A. 72, 4895–4899 (1975).

  6. 6

    Rothenberg, E. & Baltimore, D. J. Virol. 21, 168–178 (1977).

  7. 7

    Hu, S., Davidson, N. & Verma, I. M. Cell 10, 469–477 (1977).

  8. 8

    Haseltine, W. A., Kleid, D. G., Panet, A., Rothenberg, E. & Baltimore, D. J. molec. Biol. 106, 109–131 (1976).

  9. 9

    Peters, G., Harada, F., Dahlberg, J. E., Panet, A., Haseltine, W. A. & Baltimore, D. J. Virol. 21, 1031–1041 (1977).

  10. 10

    Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).

  11. 11

    Graham, F. L. & van der Eb, A. J. Virology 54, 536–539 (1973).

  12. 12

    Smotkin, D., Gianni, A. M., Rozenblatt, S. & Weinberg, R. A. Proc. natn. Acad. Sci. U.S.A. 72, 4910–4913 (1975).

  13. 13

    Smotkin, D., Yoshimura, F. K. & Weinberg, R. A. J. Virol. 20, 621–626 (1976).

  14. 14

    Rowe, W. P., Pugh, W. E. & Hartley, J. Virology 42, 1136–1139 (1970).

  15. 15

    Levy, J. A. Science 182, 1151–1153 (1973).

  16. 16

    Birnie, G. D. FEBS Lett. 26, 19–22 (1972).

  17. 17

    Manly, K. F., Smoler, D. F., Bromfeld, E. & Baltimore, D. J. Virol. 7, 106–111 (1971).

  18. 18

    Garapin, A.-C., Varmus, H. E., Faras, A. J., Levinson, W. E. & Bishop, J. M. Virology 52, 264–274 (1973).

  19. 19

    McDonell, M. W., Simon, M. N. & Studier, F. W. J. molec. Biol. 110, 119–146 (1977).

  20. 20

    Gianni, A. M. & Weinberg, R. A. Nature 255, 646–648 (1975).

  21. 21

    Fritsch, E. & Temin, H. M. J. Virol. 21, 119–130 (1977).

  22. 22

    Sharp, P. A., Sugden, B. & Sambrook, J. Biochemistry 12, 3055–3063 (1973).

  23. 23

    Laskey, R. A., Mills, A. D. & Knowland, J. S. Eur. J. Biochem. 56, 335–341 (1975).

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Rothenberg, E., Smotkin, D., Baltimore, D. et al. In vitro synthesis of infectious DNA of murine leukaemia virus. Nature 269, 122–126 (1977). https://doi.org/10.1038/269122a0

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