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Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus

Nature volume 265, pages 175–177 (1977)Cite this article

Abstract

POLY(ADP-RIBOSE) is a biopolymer which is synthesised by poly(ADP-ribose) polymerase in cell nuclei1. Its structure and physicochemical characteristics have been reviewed1. Its biological function was suggested to be related to DNA synthesis in nuclei2–4, and there are several reports on its natural occurrence5–9. We reported previously that a specific antibody to poly(ADP-ribose) could be produced in rabbits injected with a complex of poly(ADP-ribose) and methylated bovine serum albumin10. Kidwell and Mage reported on the presence of poly(ADP-ribose) in HeLa cells and the correlation of its content to cell cycles by a radioimmunoassay11. Recently we found that binding activities of the sera of patients with systemic lupus erythematosus (SLE) to 14C-poly(ADP-ribose) were higher than those of the sera of normal individuals, and we suggest that poly(ADP-ribose) as well as antibodies to poly(ADP-ribose) occur naturally in human beings. This paper reports that binding activities of the sera of SLE patients to 14C-poly(ADP-ribose) are attributable to immunoglobulins, mainly to IgG, but not to nonspecific interactions between 14C-poly(ADP-ribose) and IgG; specificity of the binding was verified by inhibition tests using unlabelled poly(ADP-ribose) and 14 related compounds.

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References

  1. Sugimura, T., Progr. Nucleic Acid Res. molec. Biol., 13, 125–151 (1973).

    Google Scholar 

  2. Burzio, L., and Koide, S. S., Biochem. biophys. Res. Commun., 40, 1013–1020 (1970).

    Article  CAS  Google Scholar 

  3. Hilz, H., and Kittler, M., Hoppe-Seyler's Z. Physiol. Chem., 352, 1693–1704 (1971).

    Article  CAS  Google Scholar 

  4. Nagao, M., Yamada, M., Miwa, M., and Sugimura, T., Biochem. biophys. Res. Commun., 48, 219–225 (1972).

    Article  CAS  Google Scholar 

  5. Doly, J., and Mandel, P., C. r. Acad. Sci. Paris, 264, 2687–2690 (1967).

    CAS  Google Scholar 

  6. Hilz, H., Bredehorst, R., Norde, S., and Kittler, M., Hoppe-Seyler's Z. Physiol. Chem., 353, 843–849 (1972).

    Article  Google Scholar 

  7. Colyer, R. A., Brudette, K. E., and Kidwell, W. R., Biochem. biophys. Res. Commun., 53, 960–966 (1973).

    Article  CAS  Google Scholar 

  8. Smith, J. A., and Stocken, L. A., Biochem. biophys. Res. Commun., 54, 297–300 (1973).

    Article  CAS  Google Scholar 

  9. Ueda, K., Omachi, A., Kawaichi, M., and Hayaishi, O., Proc. natn. Acad. Sci. U.S.A., 72, 205–306 (1975).

    Article  ADS  CAS  Google Scholar 

  10. Kanai, Y., Miwa, M., Matsushima, T., and Sugimura, T., Biochem. biophys. Res. Commun., 59, 300–306 (1974).

    Article  CAS  Google Scholar 

  11. Kidwell, W. R., and Mage, M., Biochemistry, 15, 1213–1217 (1976).

    Article  CAS  Google Scholar 

  12. Sugimura, T., Yoshimura, N., Miwa, M., Nagai, H., and Nagao, M., Archs Biochem. Biophys., 147, 660–665 (1971).

    Article  CAS  Google Scholar 

  13. Plescia, O. J., and Braun, W., Adv. Immun., 6, 231–252 (1967).

    Article  CAS  Google Scholar 

  14. Levine, L., and Stollar, B. D., Progr. Allergy, 12, 161–191 (1968).

    CAS  Google Scholar 

  15. Lacour, F., Nahon-Merlin, E., and Michelson, M., Curr. Top. Microbiol. Immun., 62, 1–39 (1973).

    CAS  Google Scholar 

  16. Botchan, M., McKenna, G., and Sharp, P. A., Cold Spring Harb. Symp. quant. Biol., 38, 383–395 (1973).

    Article  Google Scholar 

  17. Nisonoff, A., Wissler, F. C., Lipman, L. N., and Woernley, D. L., Archs Biochem. Biophys., 89, 230–244 (1960).

    Article  CAS  Google Scholar 

  18. Mandy, W. J., and Nisonoff, A., J. biol. Chem., 238, 206–213 (1963).

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, 108, Japan

    YOSHIYUKI KANAI, YOKO KAWAMINAMI, MASANAO MIWA, TAIJIRO MATSUSHIMA & TAKASHI SUGIMURA

  2. Department of Internal Medicine and Physical Therapy, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

    YASUOKI MOROI & RYUICHI YOKOHARI

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  1. YOSHIYUKI KANAI
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  2. YOKO KAWAMINAMI
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  5. TAKASHI SUGIMURA
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KANAI, Y., KAWAMINAMI, Y., MIWA, M. et al. Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus. Nature 265, 175–177 (1977). https://doi.org/10.1038/265175a0

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