Abstract
Although it carries two competent replication systems, a composite plasmid formed in vitro by linkage of the complete ColE1 and pSC101 plasmid replicons at their unique EcoRI endonuclease cleavage sites normally uses only the replication origin and functions of the ColE1 component. Restriction of ColE1 replication functions by DNA polymerase I deprivation results, however, in exclusive use of the pSC101 replication origin. When using the ColE1 replication system the composite plasmid is nevertheless incompatible with both the parent replicons. This suggests that a trans-dominant gene product is involved in plasmid incompatibility and supports negative control rather than positive control models for regulation of the initiation of DNA replication.
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References
Kornberg, A., DNA Synthesis, 176–296 (Freeman, San Francisco, 1974).
Messer, W., Dankwarth, L., Tippe-Schindler, R., Womack, J. E., and Zahn, G., ICN-UCLA Winter Conferences: DNA Synthesis and its Regulation (in the press, 1975).
Kriegstein, H. J., and Hogness, D. S., Proc. natn. Acad. Sci. U.S.A., 71, 135–139 (1974).
Kasamatsu, H., and Vinograd, J., Nature new Biol., 241, 103–105 (1973).
Rownd, R. H., Perlman, D., and Goto, N., Microbiology-1974 (edit. by Schles-singer, D.), 76–94 (American Society for Microbiology, 1974).
Crosa, J. H., Luttropp, L. K., Hefron, F., and Falkow, S., Molec. gen. Genet., 140, 39–50 (1975).
Timmis, K., Cabello, F., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 71, 4556–4560 (1974).
Kingsbury, D. T., and Helinski, D. R., Biochem. biophys. Res. Commun., 41, 1538–1544 (1970).
Bazaral, M., and Helinski, D. R., Biochemistry, 7, 3513–3519 (1968).
Cohen, S. N., and Chang, A. C. Y., Proc. natn. Acad. Sci. U.S.A., 70, 1293–1297 (1973).
Lovett, M. A., Katz, L., and Helinski, D. R., Nature, 251, 337–340 (1974).
Inselburg, J., Proc. natn. Acad. Sci. U.S.A., 71, 2256–2259 (1974).
Tomizawa, J-I., Sakakibara, Y., and Kakefuda, T., Proc. natn. Acad. Sci. U.S.A., 71, 2260–2264 (1974).
Gross, J., and Monk, M., Nature, 224, 1166–1168 (1969).
Nordström, K., Ingram, L. C., and Lundbäck, A., J. Bact., 110, 562–569 (1972).
Kool, A. J., and Nijkamp, H. J. J., J. Bact., 120, 569–578 (1974).
Uhlin, B. E., and Nordström, K., J. Bact., 124, 641–649 (1975).
Timmis, K., Cabello, F., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 72, 2242–2246 (1975).
Franklin, T. J., and Godfrey, A., Biochem. J., 94, 54–60 (1965).
Franklin, T. J., and Rownd, R. H., J. Bact., 115, 235–242 (1973).
Timmis, K., and Winkler, U., Molec. gen. Genet., 124, 207–217 (1973).
Hershfield, V., Boyer, H. W., Yanofsky, C., Lovett, M. A., and Helinski, D. R., Proc. natn. Acad. Sci. U.S.A., 71, 3455–3459 (1974).
Clewell, D. B., Yagi, Y., and Bauer, B., Proc. natn. Acad. Sci. U.S.A., 72, 1720–1724 (1975).
Echols, H., J. Bact., 85, 262–268 (1963).
Cohen, S. N., Chang, A. C. Y., Boyer, H. W., and Helling, R. B., Proc. natn. Acad. Sci. U.S.A., 70, 3240–3244 (1973).
Chang, A. C. Y., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 71, 1030–1034 (1974).
Jacob, F., Brenner, S., and Cuzin, F., Cold Spring Harb. Symp. quant. Biol., 28, 329–343 (1969).
Pritchard, R. H., Barth, P. T., and Collins, J., Symp. Soc. Gen. Microbiol., 19, 263–267 (1969).
Pritchard, R. H., Chandler, M. G., and Collins, J., Molec. gen. Genet., 138, 143–155 (1975).
Starlinger, P., and Saedler, H., Biochimie, 54, 177–185 (1972).
Hedges, R. W., and Jacob, A., Molec. gen. Genet., 132, 31–40 (1974).
Kopecko, D. J., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 72, 1373–1377 (1975).
Heffron, F., Sublett, R., Hedges, R. W., Jacob, A., and Falkow, S., J. Bact., 122, 250–256 (1975).
Bachman, B. J., Bact. Rev., 36, 525–557 (1972).
Greene, P. J., Betlach, M. D., Goodman, H. M., and Boyer, H. W., Methods in Molecular Biology (edit. by Wickner, R. B.), 9, 87–103 (Marcel Dekker, New York, 1974).
Kleinschmidt, A. K., Methods in Enzymology, XII (edit. by Colowick, S., and Kaplan, N.), 361–377 (Academic, New York, 1968).
Davis, R. W., Simon, M., and Davidson, N., Methods in Enzymology, 21, (edit. by Grossman, L., and Moldave, K.), 413–428 (Academic, New York, 1971).
Delius, H., Howe, C., and Kozinski, A. W., Proc. natn. Acad. Sci. U.S.A., 68, 3049–3053 (1971).
Cohen, S. N., Chang, A. C. Y., and Hsu, L., Proc. natn. Acad. Sci. U.S.A., 69, 2110–2114 (1972).
Timmis, K., J. Bact., 109, 12–20 (1972).
Timmis, K., and Hedges, A. J., Biochim. biophys. Acta, 262, 200–207 (1972).
Cooper, P., Genet. Res. Camb., 17, 151–159 (1971).
Datta, N., and Hedges, R. W., J. gen. Microbiol., 77, 11–17 (1973).
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Cabello, F., Timmis, K. & Cohen, S. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature 259, 285–290 (1976). https://doi.org/10.1038/259285a0
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DOI: https://doi.org/10.1038/259285a0
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