Abstract
LYMPHOCYTES are motile cells whose movement in vivo seems to be directed, yet investigators over many years have repeatedly failed to show chemotaxis of lymphocytes in vitro. There have been some recent reports that lymphocytes migrate into micropore filters towards substances placed below the filter1–3, but this migration was not found to be chemotactic3. It seemed to us that our and other people's failure to demonstrate lymphocyte chemotaxis might be because we were using the wrong population of cells. The lymphocytes which can be shown most clearly to migrate into inflammatory lesions in vivo are blast-transformed cells 4–6, and we therefore examined the migration in Boyden chambers of lymphoblasts from two sources; firstly cloned human lymphoblast cell lines maintained in continuous culture7; and secondly blast cells from the lymph nodes of CBA mice, either without deliberate sensitisation with antigen, or following exposure to the contact sensitising agent, oxazolone. Both human and mouse lymphoblasts were shown to migrate into filters towards chemoattractants. The nature of this migration is discussed below.
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RUSSELL, R., WILKINSON, P., SLESS, F. et al. Chemotaxis of lymphoblasts. Nature 256, 646–648 (1975). https://doi.org/10.1038/256646a0
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DOI: https://doi.org/10.1038/256646a0
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