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Lymphoma

Loss of heterozygosity on chromosome 6q14–q24 is associated with poor outcome in children and adolescents with T-cell lymphoblastic lymphoma

Leukemia volume 20, pages 1422–1429 (2006)Cite this article

Abstract

Deletions of chromosome 6q have been reported in several hematological malignancies, but data are not conclusive regarding their biological and prognostic impact. Therefore, we focused on pediatric patients diagnosed with T-cell lymphoblastic lymphoma (T-LBL) treated uniformly according to the NHL-BFM95 protocol. We used loss-of-heterozygosity (LOH) analysis of 25 microsatellite markers located on chromosome 6q14–q24. Fragment-length analysis was performed on ABI-PRISM3100 Genetic-Analyzer. Eligibility criterion was ⩾3 informative markers. Between April 1995 and March 2003, 185 T-LBL patients were treated according to the NHL-BFM95 protocol. Five-year event-free (EFS) and disease-free survival (DFS) were 79±3 and 87±3% (median follow-up 4.7 [1.2–10.1] years). Sixty-one patients were evaluable for LOH analysis, including 18 out of 23 patients with relapse. EFS and DFS were 67±6 and 69±6% for these 61 patients. Testing of 853 markers in the 61 patients identified the presence of LOH in 19 patients (31%): 13 of the 18 relapse patients and five of the 41 in complete remission (odds ratio 18.7, 95% confidence interval 4.7–75.3). One LOH-positive patient died from treatment-related toxicity. We conclude that LOH on chromosome 6q14–q24 may have conferred a high risk of relapse on our group of children with T-LBL treated according to the NHL-BFM95 protocol.

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Acknowledgements

We acknowledge the expert work of Ulrike Meyer, Bettina Paul, Nora Mühlegger (data management) and Edelgard Odenwald and Gabriele Buck (cytomorphology). Central histopathology and immunophenotyping of tissue samples was supported by the Kinder Krebs Initiative (KKI), Buchholz-Holm-Seppensen, Germany. The LOH analysis was performed with the support of the start-up research program of the medical faculty of the Justus-Liebig University, Giessen, Germany.

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Authors and Affiliations

  1. Department of Pediatric Hematology and Oncology, Justus-Liebig University, Giessen, Germany

    B Burkhardt, J Bruch, M Zimmermann, F Mueller, J Harbott & A Reiter

  2. Institute of Medical Biometry and Epidemiology, Philipps University Marburg, Marburg, Germany

    K Strauch

  3. Department of Hematopathology and Lymph Node Registry Kiel, Institute of Pathology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany

    R Parwaresch

  4. Department of Hematology, Oncology, and Tumor Immunology, Robert-Rössle-Clinic at the HELIOS Klinikum Berlin-Buch, Charité Medical School, Berlin, Germany

    W-D Ludwig

  5. Institute of Human Genetics, University Hospital of Schleswig-Holstein, Campus Kiel, Kiel, Germany

    L Harder

  6. Institute for Cellular and Molecular Pathology, Hannover Medical School, Hannover, Germany

    B Schlegelberger

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Burkhardt, B., Bruch, J., Zimmermann, M. et al. Loss of heterozygosity on chromosome 6q14–q24 is associated with poor outcome in children and adolescents with T-cell lymphoblastic lymphoma. Leukemia 20, 1422–1429 (2006). https://doi.org/10.1038/sj.leu.2404275

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