Abstract
Tetrahymena pyriformis, strain GL, contains two different DNA polymerase activities—one major and one minor—which can be separated from one another by gel filtration on a ‘Sephadex G–200’ column1. Exposure of Tetrahymena to various forms of radiation or thymine starvation during growth leads to a rapid increase in the total DNA polymerase activity2,3. The appearance of this new activity is dependent on RNA and protein synthesis2,3. Subsequent experiments have revealed that the rise in total DNA polymerase activity in such cells is the result solely of a marked rise (about 35-fold) in the amount of the minor of the two polymerase activities1. The activity of the major DNA polymerase remains constant. Here we show that the induced DNA polymerase activity is associated with mitochondria, implying that the induced enzyme is a mitochondrial DNA polymerase.
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WESTERGAARD, O., MARCKER, K. & KEIDING, J. Induction of a Mitochondrial DNA Polymerase in Tetrahymena. Nature 227, 708–710 (1970). https://doi.org/10.1038/227708a0
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DOI: https://doi.org/10.1038/227708a0
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