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Initiation of RNA Synthesis: the Function of σ in the Binding of RNA Polymerase to Promoter Sites

Abstract

In the presence of σ, E. coli RNA polymerase forms rifampicin resistant complexes with DNA in the absence of nucleoside triphosphates. These complexes only form at promoter sites, so the number of σ-recognized promoters per genome for several DNA phages could be determined.

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References

  1. Burgess, R. R., Travers, A. A., Dunn, J. J., and Bautz, E. K. F., Nature, 221, 43 (1969).

    Article  ADS  CAS  Google Scholar 

  2. Travers, A. A., and Burgess, R. R., Nature, 222, 537 (1969).

    Article  ADS  CAS  Google Scholar 

  3. Bautz, E. K. F., Bautz, F. A., and Dunn, J. J., Nature, 223, 1022 (1969).

    Article  ADS  CAS  Google Scholar 

  4. Dunn, J. J., and Bautz, E. K. F., Biochem. Biophys. Res. Commun., 36, 925 (1969).

    Article  CAS  Google Scholar 

  5. Lill, H., Lill, U., Sippel, A., and Hartmann, G., Proc. First Lepetit Colloquium on RNA-Polymerase and Transcription, 55 (North-Holland, Amsterdam, 1970).

    Google Scholar 

  6. Abelson, J., and Thomas, C. A., J. Mol. Biol., 18, 262 (1966).

    Article  CAS  Google Scholar 

  7. Gilbert, W., and Müller-Hill, B., Proc. US Nat. Acad. Sci., 58, 2415 (1967).

    Article  ADS  CAS  Google Scholar 

  8. Maitra, U., and Hurwitz, J., Proc. US Nat. Acad. Sci., 54, 815 (1965).

    Article  ADS  CAS  Google Scholar 

  9. Glynn, I. M., and Chappell, J. B., Biochem. J., 90, 147 (1964).

    Article  CAS  Google Scholar 

  10. Richardson, J. P., J. Mol. Biol., 21, 83 (1966).

    Article  CAS  Google Scholar 

  11. Jones, O. W., and Berg, P., J. Mol. Biol., 22, 199 (1966).

    Article  CAS  Google Scholar 

  12. Stead, N. W., and Jones, O. W., J. Mol. Biol., 26, 131 (1967).

    Article  CAS  Google Scholar 

  13. Siegel, R. B., and Summers, W. C., J. Mol. Biol. (in the press, 1970).

  14. Summers, W. C., Proc. First Lepetit Colloquium on RNA-Polymerase and Transcription, 110 (North-Holland, Amsterdam, 1970).

  15. Ptashne, M., and Hopkins, N., Proc. US Nat. Acad. Sci., 60, 1282 (1968).

    Article  ADS  CAS  Google Scholar 

  16. Salser, W., Bolle, A., and Epstein, R., J. Mol. Biol. (in the press, 1970).

  17. Bautz, E. K. F., Dunn, J. J., Bautz, F. A., Schmidt, D. A., and Mazaitis, A. J., Proc. First Lepetit Colloquium on RNA-Polymerase and Transcription, 90 (North-Holland, Amsterdam, 1970).

    Google Scholar 

  18. Travers, A. A., Nature, 224, 1168 (1969).

    Article  Google Scholar 

  19. Summers, W. C., and Siegel, R. B., Nature, 223, 1111 (1969).

    Article  ADS  CAS  Google Scholar 

  20. Riggs, A. D., Suzuki, H., and Bourgeois, S., J. Mol. Biol., 48, 67 (1970).

    Article  CAS  Google Scholar 

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BAUTZ, E., BAUTZ, F. Initiation of RNA Synthesis: the Function of σ in the Binding of RNA Polymerase to Promoter Sites. Nature 226, 1219–1222 (1970). https://doi.org/10.1038/2261219a0

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