Abstract
Two specialized transducing phages, which carry the lac operon of Escherichia coli inserted into their DNA in opposite orientations, are used as a source of complementary sequences from which to prepare pure lac operon duplex. The availability of a single pure promoter will facilitate many new experiments on genetic transcription.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).
Gilbert, W., and Muller-Hill, B., Proc. US Nat. Acad. Sci., 58, 2415 (1967).
Ptashne, M., Nature, 214, 232 (1967).
Beckwith, J. R., and Signer, E. R., J. Mol. Biol., 19, 254 (1966).
Hradecna, Z., and Szybalski, W., Virology, 32, 633 (1967).
Beckwith, J. R., Signer, E., and Epstein, W., Cold Spring Harbor Symp. Quant. Biol., 31, 393 (1966).
Campbell, A., Adv. Genet., 11, 101 (1962).
Franklin, N. C., Dove, W. F., and Yanofsky, C., Biochem. Riophys. Res. Commun., 18, 910 (1965).
Signer, E. R., Ann. Rev. Microbiol., 22, 451 (1968).
Signer, E. R., Virology, 22, 650 (1964).
Franklin, N. C., Genetics, 57, 301 (1967).
Sato, K., Nishimura, Y., Sato, M., Numich, R., and Matsushiro, A., Virology, 34, 637 (1968).
Brown, J. L., Koorajian, S., Katze, J., and Zabin, I., J. Biol. Chem., 241, 2826 (1966).
Steers, E., Craven, G. R., Anfinsen, C. G., and Bethune, J. L., J. Biol. Chem., 240, 2478 (1965).
Jones, T. H. D., and Kennedy, E. P., Fed. Proc., 27, 644 (1968).
Jones, T. H. D., and Kennedy, E. P., J. Biol. Chem. (in the press).
Riggs, A. D., and Bourgeois, S., J. Mol. Biol., 34, 361 (1968).
Taylor, K., Hradecna, Z., and Szybalski, W., Proc. US Nat. Acad. Sci., 57, 1618 (1967).
MacHittie, L. A., and Thomas, C. A., Science, 144, 1142 (1964).
Caro, L., Virology, 25, 226 (1965).
Burgess, R. R., Travers, A. A., Dunn, J. J., and Bautz, E. K. F., Nature, 221, 43 (1969).
Gillespie, D., and Spiegelman, S., J. Mol. Biol., 12, 829 (1965).
Linn, S., and Lehman, I. R., J. Biol. Chem., 240, 1294 (1965).
Kaiser, A. D., and Hogness, D. S., J. Mol. Biol., 2, 392 (1960).
Burgi, E., and Hershey, A. D., Biophys. J., 3, 309 (1963).
Studier, W. F., J. Mol. Biol., 11, 373 (1965).
Morse, M. L., Lederberg, E. M., and Lederberg, J., Genetics, 41, 142 (1965).
Matsushiro, A., Virology, 19, 475 (1963).
Bottesman, S., and Beckwith, J. R., J. Mol. Biol., 44, 117 (1969).
Studier, F. W., J. Mol. Biol., 41, 189 (1969).
Studier, F. W., J. Mol. Biol., 41, 199 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SHAPIRO, J., MACHATTIE, L., ERON, L. et al. Isolation of Pure lac Operon DNA*. Nature 224, 768–774 (1969). https://doi.org/10.1038/224768a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/224768a0
This article is cited by
-
Bacteriophages as twenty-first century antibacterial tools for food and medicine
Applied Microbiology and Biotechnology (2011)
-
Observations on the formation of clones containing araB-lacZ cistron fusions
Molecular and General Genetics MGG (1984)
-
Total synthesis of a human leukocyte interferon gene
Nature (1981)
-
Construction and characterization of a tufA-lacZ fusion coding for an E. coli EF-Tu-β-Galactosidase chimeric protein
Molecular and General Genetics MGG (1981)
-
The phage promoter responsible for the expression of the inserted β-galactosidase gene in bacteriophage λplac5
Molecular and General Genetics MGG (1980)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.