Cultivation of Rat Embryos explanted at 7.5 to 8.5 Days of Gestation

Abstract

MOUSE and rat embryos explanted at head-fold or early somite stages can be grown in culture for periods of two or three days1,2. We have now used similar methods for younger rat embryos, explanted at 7.5–8.5 days of gestation. At this stage the embryos (“egg-cylinders”) have implanted in the uterine wall (implantation in the rat occurs at about 6.5 days) and have developed an ectoplacental cone, but no organ rudiments have yet formed.

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References

  1. 1

    New, D. A. T., J. Embryol. Exp. Morphol., 17, 513 (1967).

  2. 2

    New, D. A. T., in Methods in Mammalian Embryology (Freeman, San Francisco, 1969).

  3. 3

    Goss, C. M., J. Exp. Zool., 72, 33 (1935).

  4. 4

    Daniel, J. C., in Methods in Mammalian Embryology (Freeman, San Francisco, 1969).

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NEW, D., DANIEL, J. Cultivation of Rat Embryos explanted at 7.5 to 8.5 Days of Gestation. Nature 223, 515–516 (1969). https://doi.org/10.1038/223515a0

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