Density Gradient Centrifugation of Red Blood cells carrying the Mammary Tumour Virus


SINCE the demonstration1 of mammary tumour virus (MTV) activity in the blood of mice of a high mammary cancer strain (C3H/Jax), blood-borne MTV activity has been described in other strains of mice carrying MTV2–5. It has been established that the MTV activity in blood is limited primarily to the red blood cell (RBC) fraction6. Thus we thought it interesting to determine whether all cells of the circulating RBC population carry RBC-borne MTV (R-MTV). It is not possible to determine the activity associated with the individual RBC because a large number of RBC are required for biological detection of R-MTV (our unpublished results). Some insight into this question could be gained, however, from the use of different populations of RBC.

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RITTER, R., NANDI, S. Density Gradient Centrifugation of Red Blood cells carrying the Mammary Tumour Virus. Nature 220, 403–404 (1968).

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