Isolation of a New Type of Cross Link from the Hinge Ligament Protein of Molluscs

Abstract

THE two best known elastic proteins are elastin and resilin. So far, elastin has been, isolated only from vertebrates although the presence of elastin-like material has been reported in several invertebrates1,2. Resilin has been found only in arthropods3. Apart from their amino-acid composition, resilin and elastin differ in the way they are cross linked into three dimensional networks. Thus resilin contains di- and tri-tyrosine formed oxidatively from tyrosine residues4,5 and elastin contains desmosine and isodesmosine6 together with a small amount of lysinonorleucine7, all formed from lysine residues. The presence in foetal elastin of dityrosine has recently been reported8. During an investigation of elastic proteins from other phyla, I noticed that molluscan hinge ligaments contain phenolic compounds, which in several respects resemble dityrosine (Fig. 1). One of these compounds (compound A) has now been isolated and identified as 3,3′-methylene-bistyrosine (Fig. 2). The isolation procedure and the reasons for ascribing the structure in Fig. 2 to the compound are described in this communication; a more detailed description of the properties of the compound will be published later.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Van Gansen-Semal, P., Nature, 186, 654 (1960).

    CAS  ADS  Article  Google Scholar 

  2. 2

    Elder, H. Y., and Owen, G., J. Zool., 152, 1 (1967).

    Article  Google Scholar 

  3. 3

    Andersen, S. O., and Weis-Fogh, T., Adv. Insect Physiol., 2, 1 (1964).

    CAS  Article  Google Scholar 

  4. 4

    Andersen, S. O., Biochim. Biophys. Acta, 93, 213 (1964).

    CAS  Article  Google Scholar 

  5. 5

    Andersen, S. O., Acta Physiol. Scand., 66, suppl. 263 (1966).

  6. 6

    Thomas, J., Elsden, D. F., and Partridge, S. M., Nature, 200, 651 (1963).

    CAS  ADS  Article  Google Scholar 

  7. 7

    Franzblau, C., Sinex, F. M., Farris, B., and Lampidis, R., Biochem. Biophys. Res. Commun., 21, 575 (1965).

    CAS  Article  Google Scholar 

  8. 8

    Labella, F. S., Keeley, F., Vivian, S., and Thornhill, D., Biochem. Biophy. Res. Commun., 26, 748 (1967).

    CAS  Article  Google Scholar 

  9. 9

    Silaev, A. B., Katrukha, G. S., and Stepanov, C. M., Biokhimiya, 26, 7 (1961).

    Google Scholar 

  10. 10

    Godfrey, L. E. A., Hawkins, E. G. E., and Jenkins, P. A., J. Appl. Chem., 7, 440 (1957).

    CAS  Article  Google Scholar 

  11. 11

    Brown, C. H., Quart. J. Micros. Sci., 93, 487 (1952).

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

ANDERSEN, S. Isolation of a New Type of Cross Link from the Hinge Ligament Protein of Molluscs. Nature 216, 1029–1030 (1967). https://doi.org/10.1038/2161029a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.