Abstract
SERA of patients with primary biliary cirrhosis contain autoantibodies against a non-organ specific cytoplasmic component, which are probably mitochondria1,2. A convenient way to detect these antibodies is by the fluorescent antibody technique using unfixed cryostat sections of human or rat organs. Sections of renal cortex proved to be the most suitable for the demonstration of cytoplasmic antibodies. Liver sections showed the same coarsely granular staining, but because of the uniform appearance of this tissue, it was difficult to distinguish the fluorescence obtained with the weaker sera from non-specific artefacts1.
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RÜMKE, P., BUTER, H. Detection of Antibodies against Mitochondria by the Fluorescent Antibody Technique with Smears of Rat Liver Cells. Nature 215, 1480–1481 (1967). https://doi.org/10.1038/2151480a0
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DOI: https://doi.org/10.1038/2151480a0
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