Abstract
IT is well known that glucose stimulates the release of insulin from the mammalian pancreas. It has recently been shown, in vitro, that glucose and mannose, but not a non-metabolized sugar such as galactose, will also stimulate insulin synthesis1. We have investigated the effects of glucose on the putative preceding stage, namely, the formation of new messenger RNA, using isolated islets of Langerhans from the duct-tied pancreas of the rat2,3. As the amount of tissue available for a given experiment is small (approximately 100 µg dry weight), we have investigated the incorporation of 6-14C-orotic acid into total nucleic acids of the islets.
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References
Howell, S. L., Parry, D. G., and Taylor, K. W., communication to the Medical and Scientific Section of the British Diabetic Association, 1965 October.
Keen, H., Sells, R., and Jarrett, R. J., Diabetologia, 1, 28 (1965).
Jarrett, R. J., and Keen, H., Lancet, i, 633 (1966).
Umbreit, W. W., Burns, R. H., and Stauffer, J. R., Manometric Techniques (fourth ed.) (Minneapolis, 1964.)
Wool, I. G., and Moyer, A. N., Biochim. Biophys. Acta, 91, 248 (1964).
Hall, R., and Tubmen, J., J. Biol. Chem., 240, 3132 (1965).
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JARRETT, R., KEEN, H. & TRACK, N. Glucose and RNA Synthesis in Mammalian Islets of Langerhans. Nature 213, 634–635 (1967). https://doi.org/10.1038/213634a0
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DOI: https://doi.org/10.1038/213634a0
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