Letter | Published:

Mode of Action of Hadacidin in the Growing Bacterial Cell

Naturevolume 212pages9394 (1966) | Download Citation

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Abstract

THE activities of hadacidin (N-formyl hydroxyamino-acetic acid) against the growth of human tumours1, plants2,3, cell cultures4 and as an inhibitor of chloroplast development in Euglena5 have been previously described. Isotopic experiments with Ehrlich ascites tumour cells, with cell-free tumour extracts and with intact rats led to the conclusion that the conversion of inosinic acid to adenylosuccinic acid was inhibited in these systems6. The enzyme catalysing this reaction, adenylosuccinate synthetase (inosine 5′ -phosphate: L-aspartate ligase, EC6.3.4.4), was prepared from Escherichia coli and was found to be markedly inhibited by hadacidin7. Inhibition of the enzyme was reversed by L-aspartate.

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References

  1. 1

    Gitterman, C. O., Dulaney, E. L., Kaczka, E. A., Hendlin, D., and Woodruff, H. B., Proc. Soc. Exp. Biol. and Med., 109, 852 (1962).

  2. 2

    Gray, R. A., Gauger, G. W., Dulaney, E. L., Kaczka, E. A., and Woodruff, H. B., Plant Physiol., 39, 204 (1964).

  3. 3

    Zaroogian, G. E., and Curtis, R. W., Plant and Cell Physiol., 5, 291 (1964).

  4. 4

    Neuman, R. E., and Tytell, A. A., Proc. Soc. Exp. Biol. and Med., 112, 57 (1963).

  5. 5

    Mego, J. L., Biochem. Biophys. Acta, 79, 221 (1964).

  6. 6

    Shigeura, H. T., and Gordon, C. N., J. Biol. Chem., 237, 1932 (1962).

  7. 7

    Shigeura, H. T., and Gordon, C. N., J. Biol. Chem., 237, 1937 (1962).

  8. 8

    Shigeura, H. T., and Gordon, C. N., Cancer Res., 22, 1356 (1962).

  9. 9

    Stapley, E. O., Gitterman, C. O., Taussig, F., and Miller, A. K. (unpublished results).

  10. 10

    Aoki, R., Momose, H., Kondo, Y., Muramatsu, N., and Tsuchiya, Y., J. Gen. App. Microbiol. (Japan), 9, 387 (1963).

  11. 11

    Demain, A. L., Arch. Biochem. Biophys., 108, 403 (1964).

  12. 12

    Demain, A. L., Burg, R. B., and Hendlin, D., J. Bacteriology, 89, 640 (1965).

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Affiliations

  1. Merck, Sharp and Dohme Research Laboratories, Division of Merck and Co., Inc., Rahway, New Jersey

    • ARNOLD L. DEMAIN

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https://doi.org/10.1038/212093a0

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