Abstract
THE glycoprotein-rich seromucoid fraction1 is isolated by first precipitating most serum proteins with perchloric acid—seromucoid being precipitated from the filtrate with phosphotungstic acid. The solubility of certain glycoproteins in perchloric acid is assumed due to covalently bound hexose, hexosamine, fucose and sialic acid residues. Thus, seromucoid contains perchloric acid-soluble orosomucoid, Zn-α2-glycoprotein, haemopexin, the haptoglobins and 3.5 S α1-glycoprotein2 with total carbohydrate and sialic acid contents above 8.0 and 3.3 per cent respectively. Insoluble glycoproteins, absent from seromucoid, include α2-macroglobulins, ceruloplasmin and transferrin with less carbohydrate content. Seromucoid also contains small amounts of co-precipitated albumin, pre-albumin and γ-globulins which, when pure, are perchloric acid-insoluble.
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ANDERSON, A. Factors affecting the Amount and Composition of the Serum Seromucoid Fraction. Nature 208, 491–492 (1965). https://doi.org/10.1038/208491a0
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DOI: https://doi.org/10.1038/208491a0
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