Haemagglutinating, Precipitating and Lymphocyte-stimulating Factors of Phytohaemagglutinin

Abstract

EXTRACTS from certain seeds of the Leguminosae are known to possess interesting biological properties. An extract of Phaseola vulgaris, phytohaemagglutinin (PHA), agglutinates red and white blood cells1,2 and tumour cells3, and indiscriminately stimulates blood lymphocytes cultured in vitro to undergo blastic transformation and increased mitogenic activity4. Since lymphocytes of a sensitized donor are known to be stimulated in the same manner when exposed to a previously encountered antigen5,6, the possibility of PHA being a universal antigen has been considered7. Recently it has been noted by ourselves and others8,9 that PHA likewise forms a precipitate indiscriminately with serum. Our demonstration of this ability of PHA to form a precipitin line with human serum and numerous animal sera appeared to support the concept of its being a ubiquitous antigen. If this were a valid concept, however, treatment of PHA to remove its precipitating activity would entail loss of antigenicity and might be expected to impair the lymphocyte-stimulating factor as well.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Rigas, D. A., and Osgood, E. E., J. Biol. Chem., 212, 607 (1955).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. 2

    Nungester, W. J., and Halsema, G. V., Proc. Soc. Exp. Biol. and Med., 83, 863 (1953).

    CAS  Article  Google Scholar 

  3. 3

    Tunnis, M., J. Immunol., 92, 864 (1964).

    Google Scholar 

  4. 4

    Nowell, P. C., Cancer Res., 20, 462 (1960).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. 5

    Pearmain, G., Lycette, R. R., and Fitzgerald, P. H., Lancet, i, 637 (1963).

  6. 6

    Holland, P., and Mauer, A. M., Lancet, ii, 1368 (1964).

  7. 7

    Lycette, R. R., and Pearmain, G. E., Lancet, ii, 386 (1963).

  8. 8

    Nakamura, S., Tanaka, K., and Murakawa, S., Nature, 188, 144 (1960).

    ADS  CAS  Article  Google Scholar 

  9. 9

    Beckman, L., Nature, 195, 582 (1962).

    ADS  CAS  Article  Google Scholar 

  10. 10

    Boyden, S. V., J. Exp. Med., 93, 107 (1951).

    CAS  Article  Google Scholar 

  11. 11

    Crowle, A. J., J. Lab. Clin. Med., 52, 784 (1958).

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

HOLLAND, N., HOLLAND, P. Haemagglutinating, Precipitating and Lymphocyte-stimulating Factors of Phytohaemagglutinin. Nature 207, 1307–1308 (1965). https://doi.org/10.1038/2071307a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.