Abstract
WE have shown1 that β-hydroxybutyrate does not induce secretion of insulin in vitro as does glucose or citrate. Newsholme, Randle and Manchester2 have observed a diminution of uptake of glucose (perfused heart) in the presence of β-hydroxybutyrate, acetoacetate and octonoate. They conclude that these substrates inhibit uptake of glucose by inhibiting the intracellular phosphorylation of glucose by hexokinase and the phosphofructokinase reaction. According to Passonneau and Lowry3 phosphofructokinase is responsive to the balance between ∼P expenditure and ∼P formation. In view of those results we decided to ascertain the effect of glucose on insulin secretion in vitro by rabbit pancreas pre-treated with β-hydroxybutyrate.
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References
Candela, J. L. R-, Candela, R. R-., and Martin-Hernandez, D., Nature, 195, 711 (1962).
Newsholme, E. A., Randle, P. J., and Manchester, K. L., Nature, 193, 270 (1962).
Passonneau, J. V., and Lowry, O. H., Biochem. Biophys. Res. Commun., 13, 372 (1963)
Grodsky, G., and Tarver, H., Nature, 177, 223 (1956).
de la Loma, J. L., Experimentación Agricola, Hispano-American Edition (Mexico).
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An erratum to this article is available at https://doi.org/10.1038/2041141e0
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ROJAS, J., MENESES, P. & R-CANDELA, J. Effect of Glucose on Insulin Secretion in vitro by Rabbit Pancreas pre-incubated with β-Hydroxybutyrate. Nature 203, 758 (1964). https://doi.org/10.1038/203758a0
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DOI: https://doi.org/10.1038/203758a0
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