Abstract
THE principle of this method is that a relatively voluminous sample (50–100 µl.) is easily applied over a large area of chromatographic substrate and can then be swept into a narrow band by an advancing solvent front. This narrow band is used as the starting line for conventional thin-layer chromatography. The width of the band compares favourably with the diameter of spots applied by the time-consuming, conventional method of repeated application of microlitre amounts. We have used the method for the chromatography of amino-acids in aqueous solvents on 250-µ thick layers of both silica and cellulose, and the method should have general applicability.
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References
Bush, I. E., The Chromatography of Steroids (Pergamon Press, Oxford and New York, 1961).
Yphantis, D. A., Ann. N.Y. Acad. Sci., 88, 586 (1960).
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FESSLER, J., GALLEY, H. Thin-layer Chromatography of Relatively Voluminous Samples. Nature 201, 1056 (1964). https://doi.org/10.1038/2011056a0
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DOI: https://doi.org/10.1038/2011056a0
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