Abstract
DISK electrophoresis, as originated by Ornstein and Davis1, provides a delicate analytical technique for the resolution of protein mixtures, but it requires nearly 2 h to complete a run. The formation of two acrylamide gels which merely prevent convection and take no direct part in the separation occupies some forty minutes. Barka avoided this step by simply layering the proteins in an appropriate buffer over the running gel2. Reisfeld3 added sucrose to the sample mixture so that the density gradient diminished diffusion, but it has been reported that this manœuvre necessitates a reduced voltage during electrophoresis4.
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References
Ornstein, L., and Davis, B. J., Disc. Electrophoresis (preprinted by Distillation Products Industries, Eastman Kodak Co., 1962).
Barka, T., J. Histochem. Cytochem., 9, 542 (1961).
Reisfeld, R. A., Lewis, U. J., and Williams, D. E., Nature, 195, 281 (1962).
Fox, D. J., Thurman, D. A., and Bouleter, D., Biochem. J., 87, 29, P (1963).
Bangham, A. D., and Lehmann, H., Nature, 181, 267 (1958).
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BROOME, J. A Rapid Method of Disk Electrophoresis. Nature 199, 179–180 (1963). https://doi.org/10.1038/199179b0
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DOI: https://doi.org/10.1038/199179b0
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