Abstract
A METHOD for the separation of iodide from iodotyrosines extracted with Blau's alkaline reagent (4 N sodium hydroxide in 5 per cent sodium carbonate) from n-butanol extracts of thyroid hydrolysates by means of modified charcoal was previously described1. This method suffered from the following shortcomings: removal of traces of butanol from the alkali wash was quite difficult, and, since the butanol interfered with the adsorption of the iodotyrosines on the charcoal, repeated treatment of the alkali with the charcoal was required; the adsorbed iodotyrosines could be eluted from the charcoal in toto but could not be separated into mono- and di-iodotyrosines. This communication deals with further developments of the method to overcome the foregoing handicaps.
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References
Posner, I., and Pimentel, E., Nature, 195, 1097 (1962).
Roche, J., Jutisz, M., Lissitzky, S., and Michel, R., Biochim. Biophys. Acta, 7, 257 (1951).
Bowden, C. H., Maclagan, N. F., and Wilkinson, J. H., Biochem. J., 59, 93 (1955).
Posner, I., J. Lab. Clin. Med., 57, 314 (1961).
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POSNER, I., PIMENTEL, E. Further Separation of Iodine, Monoiodotyro-sine and Diiodotyrosine by means of Modified Charcoal. Nature 199, 177–178 (1963). https://doi.org/10.1038/199177a0
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DOI: https://doi.org/10.1038/199177a0
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