Abstract
FROM small-scale, pure cultures of several rumen oligotrichs, including Eremoplastron bovis (Eudiplodinium neglectum, Dogiel1), Hungate2 prepared extracts containing cellulase and cellobiase. The extracts were not tested for other carbohydrases and appear to represent the only direct evidence for cellulolytic enzymes in rumen protozoa, apart from a recent observation of cellodextrinase in the extract from mixed Entodinium species3. Cultures of several oligotrich species were recently established4 in this laboratory, enabling us to prepare 2–3 g (wet wt.) of cells of Eremoplastron bovis for a more detailed examination of its carbohydrases.
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References
Dogiel, V. A., Arch. Protistenk., 59, 1 (1927).
Hungate, R. E., Biol. Bull., 83, 303 (1942); 84, 157 (1943).
Bailey, R. W., and Clarke, R. T. J., Nature, 198, 787 (1963).
Clarke, R. T. J., J. Gen. Microbiol. (in the press).
Fernley, H. N., Biochem. J., 87, 90 (1963).
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BAILEY, R., CLARKE, R. Carbohydrases of the Rumen Oligotrich Eremoplastron bovis. Nature 199, 1291–1292 (1963). https://doi.org/10.1038/1991291b0
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DOI: https://doi.org/10.1038/1991291b0
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