Abstract
WHEN ethyl pyruvate and 4,5-diaminopyrimidine were refluxed in water, between pH 1 and 3, a solid of molecular weight about 320 was formed which was seen as two spots1 after dissolution in 0.1 N sodium hydroxide and chromatography on a sheet of Whatman No. 1 paper in water at pH 5.5 or 8.5. The spots (Fig. 1A) had RF values of 0.55 and 0.65 respectively and appeared identical when viewed in ultra-violet light. When eluted, and rechromatographed, each spot generated only one spot, which corresponded in RF to the spot from which it was eluted (Fig. 1B and C).
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References
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ALBERT, A., SERJEANT, E. Resolution of a Racemic Pteridine during Paper Chromatography. Nature 199, 1098–1099 (1963). https://doi.org/10.1038/1991098a0
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DOI: https://doi.org/10.1038/1991098a0
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