Letter | Published:

Binding of RP2-L—a Nuclear Protein of Neoplastic Tissues—to Deoxyribonucleic Acid

Naturevolume 192pages870871 (1961) | Download Citation



RECENT work in this laboratory has indicated that the incorporation of radioactive amino-acids into nuclear proteins, especially the acid-soluble nuclear proteins, represents an important metabolic pathway in transplantable rat tumours1–3. The acid-soluble nuclear proteins obtained from rats injected with L-lysine-U-14C were chromatographed on carboxymethyl-cellulose columns4 after the acid extracts had been dialysed against 0.5 N acetic acid. The cellulose columns (sodium form) had been equilibrated against 0.05 M sodium acetate buffer, pH 4.0. Gradient elution was used with N formic acid followed by 8 N formic acid. All procedures were carried out at 4° C. The resolving power of this method provided specific chromatographic patterns for protein distribution and distribution of radioactivity for the acid-soluble nuclear proteins of the Walker tumour and various tissues of the tumour-bearing rat. The chromatograms of the acid-soluble nuclear proteins from the various non-tumour tissues contained one to three radioactive peaks with relatively low specific activities. The chromatograms of the nuclear extracts of the Walker tumour, and a number of other malignant tumours, contained a radioactive peak which was not found in any of the growing or non-growing4,5 non-tumour tissues studied.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Busch, H., Davis, J. R., and Anderson, D. C., Cancer Research, 18, 926 (1958).

  2. 2

    Busch, H., Davis, J. R., Honig, G. R., Anderson, D. C., Nair, P. V., and Nyhan, W. L., Cancer Research, 19, 1030 (1959).

  3. 3

    Davis, J. R., and Busch, H., Cancer Research, 18, 718 (1958).

  4. 4

    Davis, J. R., and Busch, H., Cancer Research, 19, 1157 (1959).

  5. 5

    Davis, J. R., and Busch, H., Cancer Research, 20, 1208 (1960).

  6. 6

    Busch, H., Starbuck, W. C., and Davis, J. R., Cancer Research, 19, 684 (1959).

  7. 7

    Ahlström, L., Arkiv Kemi Mineral. Geol., 24A, 31 (1947).

  8. 8

    Gregoire, J., Bull. Soc. Chim. Biol., 36, 15 (1954).

  9. 9

    Crampton, C. F., Lipshitz, R., and Chargaff, E., J. Biol. Chem., 206, 499 (1954).

  10. 10

    Bakay, B., Kolb, J. J., and Toennies, G., Arch. Biochim. Biophys., 58, 144 (1955).

Download references

Author information


  1. Department of Pharmacology and Biochemistry, Baylor University College of Medicine, Houston, 25, Texas



  1. Search for PAUL BYVOET in:

  2. Search for HARRIS BUSCH in:

About this article

Publication history

Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.