Letter | Published:

A Sealing Compound for use in Biological Work

Naturevolume 191page410 (1961) | Download Citation



IN the course of rhizosphere studies, it was desired to measure continuously the respiration of intact sterile roots during the growth and development of a variety of plants. To separate the photosynthetic and respiratory activities of the portions above ground from the respiration of the roots, a material was required to prevent diffusion of gases from one zone to the other. The material had to fulfil several requirements in order to be effective : (1) be impermeable to gases and water ; (2) be non-toxic to plants ; (3) cause no physical damage to plants ; (4) be sufficiently pliable to permit normal enlargement of stems ; (5) withstand a continuous positive pressure of 0.5–1.0 lb./in.2; (6) resist microbial and environmental deterioration; (7) be autoclavable; (8) be easily handled and applied; (9) be relatively inexpensive.

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  1. 1

    Brun, W. A., Plant Physiol. (in the press).

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  1. Central Research Laboratories, United Fruit Co., Norwood, Massachusetts

    • G. STOTZKY
    • , W. CULBRETH
    •  & L. B. MISH


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