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Determination by Mass Spectrometry of the Structure of Proline Derivatives from Apples

Naturevolume 191pages380381 (1961) | Download Citation



Hulme1 and Urbach2 independently isolated a new amino-acid from apples, and the isomeric structures I2 and II1,3 were proposed for these substances on the basis of elemental analysis, colour reactions and chromatographic properties1–3. The infra-red spectra of these compounds proved to be superimposable, and this indicated that the compounds were identical, but it did not permit a decision between the alternative structures3.

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  1. 1

    Hulme, A. C., Nature, 174, 1055 (1954).

  2. 2

    Urbach, G., Nature, 175, 170 (1955).

  3. 3

    Hulme, A. C., and Steward, F. C., Nature, 175, 171 (1955).

  4. 4

    Biemann, K., Seibl, J., and Gapp, F., Biochem. Biophys. Res. Comm., 1, 307 (1959).

  5. 5

    Biemann, K., Lioret, C., Asselineau, J., Lederer, E., and Polonsky, J., Biochim. Biophys. Acta, 40, 369 (1960).

  6. 6

    Biemann, K., and Vetter, W., Biochem. Biophys. Res. Comm., 2, 93 (1960).

  7. 7

    Burroughs, L. F., Dalby, S., Kenner, G. W., and Sheppard, R. C., Nature, 189, 394 (1961).

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  1. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts

    • K. BIEMANN
    •  & G. G. J. DEFFNER
  2. Department of Botany, Cornell University, Ithaca, New York

    • F. C. STEWARD


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