Cytochemical Changes in Human Amnion Cells infected with Psittacosis Virus


BEDSON et al. 1 described the developmental forms of psittacosis virus using conventional staining methods and light microscopy. Subsequent studies by others, who used phase, ultra-violet2, and electron3 microscopy, and fluorescein-tagged antibody4, helped to describe the complex nature of the replication-cycle. In this work, we used Feulgen staining5 and fluorescence microscopy with acridine orange dye to demonstrate cytochemical changes in tissue cultures infected with psittacosis virus. Immunocytochemical changes were followed by fluorescent-antibody techniques6. Pretreatment of infected cells with ribonuclease was also used in these staining procedures.


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POLLARD, M., STARR, T., MOORE, R. et al. Cytochemical Changes in Human Amnion Cells infected with Psittacosis Virus. Nature 188, 770 (1960).

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