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Lysosomal-like Particles in the Rat Mammary Gland

Nature volume 188, pages 318–320 (1960)Cite this article

Abstract

DE DUVE et al. 1 have described the occurrence in rat liver of a new type of cytoplasmic particle, the lysosome, which was characterized by the following properties: (1) A distinct enzymic composition, containing a number of hydrolytic enzymes (acid phosphatase, β-glucuronidase, acid ribonuclease and acid deoxyribonuclease). (2) They can be isolated as a distinct intermediate fraction, between the mitochondria and microsomes, by differential centrifugation. (3) When isolated undamaged, the lysosomes show a low level of enzyme activity, but when the membrane is ruptured by freezing, and thawing, or by the addition of non-ionic detergents such as ‘Triton-X-100’, there is a considerable increase in the activity of the enzymes.

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Author notes

  1. T. F. SLATER: Beit Memorial Fellow.

Authors and Affiliations

  1. Department of Biochemistry, University College, London, W.C.1

    A. L. GREENBAUM, T. F. SLATER & D. Y. WANG

Authors
  1. A. L. GREENBAUM
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  2. T. F. SLATER
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  3. D. Y. WANG
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GREENBAUM, A., SLATER, T. & WANG, D. Lysosomal-like Particles in the Rat Mammary Gland. Nature 188, 318–320 (1960). https://doi.org/10.1038/188318a0

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