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Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose

Nature volume 187, pages 1127–1128 (1960)Cite this article

Abstract

THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations1. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day2.

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References

  1. Milstone, J. H., Proc. Soc. Exp. Biol. and Med., 103, 361 (1960).

    Article  CAS  Google Scholar 

  2. Sober, H. A., Gutter, F. J., Wyckoff, M. M., and Peterson, E. A., J. Amer. Chem. Soc., 78, 756 (1956).

    Article  CAS  Google Scholar 

  3. Milstone, J. H., J. Gen. Physiol., 42, 665 (1959).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).

    CAS  PubMed  Google Scholar 

  5. Mellanby, J., and Pratt, C. L. G., Proc. Roy. Soc., B, 128, 201 (1939).

    Article  ADS  Google Scholar 

  6. Sherry, S., and Troll, W., J. Biol. Chem., 208, 95 (1954).

    CAS  PubMed  Google Scholar 

  7. Milstone, J. H., J. Gen. Physiol., 38, 757 (1955).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Macfarlane, R. G., J. Physiol., 106, 104 (1947).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Arscott, P. M., Koppel, J. L., and Olwin, J. H., Nature, 183, 753 (1959).

    Article  ADS  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Yale University School of Medicine, New Haven, Connecticut

    J. H. MILSTONE

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  1. J. H. MILSTONE
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MILSTONE, J. Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose. Nature 187, 1127–1128 (1960). https://doi.org/10.1038/1871127a0

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