Letter | Published:

Failure to Recover Infective ‘Ribonucleic Acid’ from Myxovirus Preparations

Abstract

IT now seems clear that preparations of infective ‘ribonucleic acid’ can be obtained from crude preparations of many animal viruses, although Colter et al. have reported failure with Bunyamwera virus preparations1. The technique used in most cases is similar to the treatment introduced by Gierer and Schramm2, namely, exposure of the virus preparation to concentrated phenol. Recognition that the infectivity of the product of phenol treatment is due to a component other than intact virus is based on such criteria as susceptibility to ribonucleases, stability in different media and rate of sedimentation in the ultracentrifuge1,2.

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References

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    Colter, J. S., Bird, H. H., and Brown, R. A., Nature, 179, 859 (1957). Wecker, E., and Schäfer, W., Z. Naturforsch., 12b, 415 (1957). Colter, J. S., Bird, H. H., Moyer, A. W., and Brown, R. A., Virology, 4, 522 (1957). Brown, F., Sellers, R. F., and Stewart, D. L., Nature, 182, 535 (1958). Cheng, P. Y., Nature, 181, 1800 (1958). Ada, G. L., and Anderson, S. G., Nature, 183, 799 (1959).

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    Gierer, A., and Schramm, G., Nature, 177, 702 (1956).

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    Ada, G. L., and Perry, B. T., Aust. J. Exp. Biol., 32, 453 (1954). Burke, D. C., Isaacs, A., and Walker, J., Biochim. Biophys. Acta, 26, 576 (1957).

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    Burnet, F. M., and Lind, P. E., Aust. J. Exp. Biol., 32, 133 (1954). Baron, S., and Jensen, K. E., J. Exp. Med., 102, 677 (1955). Gotlieb, T., and Hirst, G. K., Virology, 2, 235 (1956). Lind, P. E., and Burnet, F. M., Aust. J. Exp. Biol., 35, 531 (1957).

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