Abstract
IN assaying antibiotic substances by any of the well-known plate diffusion methods it is necessary to dilute the unknown solution to varying degrees, depending on the original concentration. In addition to minimizing the possible disturbing influence of interfering substances and the pH of the original sample in the assay plate, this procedure has the practical convenience of providing inhibitory zones of a suitable size-range needed for a satisfactory assay. However, if one could run good assays on the original concentrated samples without resorting to serial dilution it would simplify the assay procedures. We have found that such a step is possible with the use of a micrometer syringe which would enable one to spot micro-volumes directly on the assay plate with great accuracy. These minute volumes dry rapidly and form discrete spots, giving uniformly circular inhibition zones after allowing proper diffusion and incubation periods.
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References
“The Compilation of Tests and Methods of Assay for Antibiotic Drugs”, U.S. Federal Security Agency, Food and Drug Administration, 1 (1954).
Miyamura, S., Antibiotics and Chemotherapy, 6, 658 (1956).
Glynn Jones, G. D., and Lowe, H., J. Lab. Practice, 5, 69 (1956).
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IYENGAR, M. Micro-volume Drop-plate Assay of Antibiotics. Nature 183, 684 (1959). https://doi.org/10.1038/183684a0
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DOI: https://doi.org/10.1038/183684a0
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