Detection of Triterpenoid Glycosides on Paper Chromatograms


IN the course of our investigations of Echinocystis lobata seeds, it became desirable to locate triterpenoid glycosides on paper chromatograms by means of a colour reaction. A modification of the Liebermann–Burchard test described by Neher and Wettstein1 was unsatisfactory for this purpose. We found that the original Liebermann–Burchard reaction applied to paper chromatograms with comparatively little alterations gave more satisfactory results, permitting a reliable detection of the spots.

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  1. 1

    Neher, R., and Wettstein, A., Helv. Chim. Acta, 34, 2283 (1951).

  2. 2

    Partridge, S. M., Biochem. J., 42, 238 (1948).

  3. 3

    Brieskorn, C. H., Planta Medica, 2, 35 (1954).

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