Abstract
ELECTROPHORESIS of protein mixtures in agar gels has been described by Gordon et al. 1. It has been developed by Grabar and Williams and used in their immuno-electrophoretic method2. The latter, thanks to the specificity and sensitivity of immuno-chemical reactions, permits the detection of the presence even of the smallest amounts of components in a protein mixture and permits defining them by their electrophoretic mobilities. The agar gels can be dried and stained3, as in paper electrophoresis. However, the use of agar has one disadvantage : the samples are usually introduced in the gel, mixed with melted agar, thus being heated to 40°–45° C. Thus there is a constant danger of denaturing certain more sensitive protein components. A possible interaction of agar with some of the substances analysed cannot be excluded. Finally, it was of interest to find a gel from which the electrophoretically separated components could be extracted and estimated quantitatively.
Similar content being viewed by others
Article PDF
References
Gordon, A. H., Keil, B., and Šebesta, K., Nature, 164, 498 (1949). Gordon, A. H., Keil, B., Šebesta, K., Knessl, O., and Sorm, F., Coll. Trav. Chim. Tchecosl., 15, 1 (1950).
Grabar, P., and Williams, jun., C. A., Biochim. Biophys. Acta, 17, 67 (1955). Williams, jun., C. A., and Grabar, P., J. Immunol., 74, 158, 397, 404 (1955).
Uriel, J., and Scheidegger, J. J., Bull. Soc. Chim. Biol., 37, 165 (1955). Uriel, J., and Grabar, P., Ann. Inst. Pasteur [90, 427 (1956)].
Consden, R., and Powell, M. N., J. Clin. Path., 8, 150 (1955).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GRABAR, P., NOWINSKI, W. & GENEREAUX, B. Use of Pectin in Gel Electrophoresis. Nature 178, 430 (1956). https://doi.org/10.1038/178430a0
Issue Date:
DOI: https://doi.org/10.1038/178430a0
This article is cited by
-
The Laboratory Technology of Discrete Molecular Separation: The Historical Development of Gel Electrophoresis and the Material Epistemology of Biomolecular Science, 1945–1970
Journal of the History of Biology (2009)
-
Die Immunoelektrophorese in Agar-Gel
Experientia (1957)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.