Electrophoresis of Human Red-Cell Stroma

Abstract

IN an attempt to provide a chemical basis for an understanding of the morphological appearance of human red cells and of the changes in red-cell morphology in various hæmatological disorders, we have fractionated red-cell stroma preparations by electrophoresis. Three lipid-containing protein fractions have been separated, two of which migrate readily in veronal buffer at pH 8.6 (µ = 0.1). Red-cell stroma was prepared initially in the manner described by us¹, but in addition was washed twice with 0.1 M potassium dihydrogen phosphate and three times with distilled water. A stromal extract obtained with 0.2 M ammonium hydroxide and containing 18 µgm. of tyrosine equivalents of protein² was then subjected to paper electrophoresis³.