Abstract
THE paper chromatography of steroids, for example, cortical steroids, has already been investigated by Zaffaroni et al.1 and by Bush2. Although the separation on paper of the natural œstrogens free or in compounds has already been investigated by Bush3, Heftmann4, Nyc et al.5 and Boscott6, a satisfactory separation of the three natural steroids on non-treated paper has, to our knowledge, not yet been published. The method described below gives excellent results. It has been especially devised for separating and purifying the small amounts of œstrogens normally present in biological fluids.
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References
Zaffaroni, A., Burton, R. B., and Keutman, E. H., Science, 111, 6 (1950).
Bush, I. E., Biochem. J., 50, 370 (1952).
Bush, I. E., Nature, 166, 445 (1950).
Heftmann, E., Science, 111, 571 (1950).
Nyc, J. F., Garst, J. B., Friedgood, H. B., and Marion, D. M., Arch. Biochem., 29, 219 (1950).
Boscott, R. J., Biochem. J., 48 (1951) and 49 (1951).
Heusghem, C., Bull. Soc. Chim. Biol., 31, 1114 (1949).
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HEUSGHEM, C. Paper Partition Chromatography of Natural Œstrogens. Nature 171, 42–43 (1953). https://doi.org/10.1038/171042b0
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DOI: https://doi.org/10.1038/171042b0
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