Abstract
THE separation of several non-volatile carboxylic acids by paper chromatography has been described by Lugg and Overell1,2. These workers found that it was impossible to carry out the separation with neutral solvents because the ionization of the acids caused the spots to ‘tail’. They overcame the difficulty by swamping both the stationary and mobile phases with formic or acetic acid. Removal of the swamping acid was then a necessary and sometimes tedious operation before the non-volatile acids could be revealed by spraying with an acid-base indicator.
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References
Lugg, J. W. H., and Overell, B. T., Nature, 160, 87 (1947).
Lugg, J. W. H., and Overell, B. T., Aust. J. Sci. Research, A, 1, 98 (1948).
Brown, F., and Hall, L. P., Nature, 166, 66 (1950).
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Williams, R. J., and Kirby, H., Science, 107, 481 (1948).
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BROWN, F. Application of Paper Chromatography to the Separation of Non-Volatile Carboxylic Acids. Nature 167, 441 (1951). https://doi.org/10.1038/167441a0
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DOI: https://doi.org/10.1038/167441a0
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