Abstract
THE tissue test method1, 2 has proved useful in the diagnosis of mineral element deficiencies in a variety of crop plants. Using this technique, however, it has not been possible hitherto to detect deficiency-levels of manganese in plant extracts, due to the fact that Morgan's reagent (100 gm. sodium acetate and 30 ml. glacial acetic acid per 1 litre at pH. 4.8) does not extract sufficient manganese from healthy or manganese-deficient tissues to be detected by the formaldoxime test2. The minimum concentration for which this reagent can be used is 1 part in 5 million. The value of the latter reagent in detecting excessive concentrations of manganese in plant tissue extracts has been reported previously2, 3, 4.
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NICHOLAS, D. Detection of Manganese Deficiency in Plants by Tissue Tests, Using Tetramethyldiaminodiphenylmethane. Nature 157, 696 (1946). https://doi.org/10.1038/157696b0
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DOI: https://doi.org/10.1038/157696b0
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