This Month |
Featured
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Article |
Targeted deubiquitination rescues distinct trafficking-deficient ion channelopathies
Engineered deubiquitinases (enDUBs) use nanobodies to bring deubiquitinases to target proteins for selective ubiquitin chain removal. enDUBs can rescue the functional expression of mutant ion channels like those responsible for cystic fibrosis.
- Scott A. Kanner
- , Zunaira Shuja
- & Henry M. Colecraft
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Tools in Brief |
Capturing ubiquitin-binding interactions
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This Month |
Koraljka Husnjak
A “simple but a bit crazy idea” to tag ubiquitin and practice multilingual, multidisciplinary proteomics.
- Vivien Marx
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Article |
Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
A lysine-less, internally affinity-tagged ubiquitin construct is deployed to discover linear polyubiquitinated substrates via a mass-spectrometry-based proteomics approach.
- Katarzyna Kliza
- , Christoph Taumer
- & Koraljka Husnjak
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Article |
A bacterial genetic selection system for ubiquitylation cascade discovery
An Escherichia coli-based genetic selection system, constructed from a split antibiotic resistance protein individually tethered to ubiquitin and a target protein, helps discover ubiquitin cascade pathway interactions.
- Olga Levin-Kravets
- , Neta Tanner
- & Gali Prag
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Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation
Two methods for identifying protein isoforms that are concurrently phosphorylated and ubiquitylated are applied in yeast to identify phosphorylation sites that regulate ubiquitin proteasome–mediated proteome degradation.
- Danielle L Swaney
- , Pedro Beltrao
- & Judit Villén
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Tools in Brief |
Profiling ubiquitin-like modifications
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Article |
Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling
Sensor proteins that exploit principles of linkage-specific avidity reveal topology-related functions of polyubiquitin in diverse cell types and pathways.
- Joshua J Sims
- , Francesco Scavone
- & Robert E Cohen
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Article |
A photoconvertible reporter of the ubiquitin-proteasome system in vivo
A photoconvertible reporter of the ubiquitin-proteasome system permits detection of its activity independent of protein synthesis and is applied to study cell type– and age-specific protein degradation in living Caenorhabditis elegans.
- Geert Hamer
- , Olli Matilainen
- & Carina I Holmberg
Henry Colecraft