Research Highlights |
Featured
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Tools in Brief |
Capturing ubiquitin-binding interactions
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Research Highlights |
A proteome-wide view of thermal stability
Proteome thermal stability is probed using limited proteolysis and mass spectrometry.
- Allison Doerr
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Correspondence |
Protein digestion priority is independent of protein abundances
- Mingliang Ye
- , Yanbo Pan
- & Hanfa Zou
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Advertising Feature: Application Note |
Trypsin/Lys-C protease mix for enhanced protein mass spectrometry analysis
- Sergei Saveliev
- , Mark Bratz
- & Marjeta Urh
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Tools in Brief |
Protein degradation sensors
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Article |
iGLuc: a luciferase-based inflammasome and protease activity reporter
A reporter of caspase-1 activity based on Gaussia luciferase—iGLuc—reveals inflammasome activation in mouse cells and in vivo. iGLuc forms nonluminescent aggregates in cells that can be cleaved by specific proteases and, consequently, lead to luciferase activity. The design principle is applied to build reporters for several other proteases.
- Eva Bartok
- , Franz Bauernfeind
- & Veit Hornung
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Brief Communication |
A protease for 'middle-down' proteomics
A rare cutting protease that creates large peptides is well suited for differentiating protein isoforms and detecting combinations of post-translational modifications by tandem mass spectrometry.
- Cong Wu
- , John C Tran
- & Neil L Kelleher
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Research Highlights |
Degrading proteins with intracellular antibodies
Researchers develop a genetic method for degradation of GFP protein fusions in cells and living flies.
- Erika Pastrana
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Research Highlights |
Regulation through degradation
Two groups present small molecule–induced degradation systems for controlling protein function in living systems.
- Allison Doerr
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Brief Communication |
Proteome-wide analysis of protein carboxy termini: C terminomics
A method to analyze the sequence of C-terminal peptides using a combination of a specific enrichment approach and mass spectrometry is described, allowing the study of C-terminal proteolytic processing on a global scale. Also in this issue, Van Damme et al. describe a related method for simultaneous C- and N-terminal peptide analysis.
- Oliver Schilling
- , Olivier Barré
- & Christopher M Overall
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Article |
A photoconvertible reporter of the ubiquitin-proteasome system in vivo
A photoconvertible reporter of the ubiquitin-proteasome system permits detection of its activity independent of protein synthesis and is applied to study cell type– and age-specific protein degradation in living Caenorhabditis elegans.
- Geert Hamer
- , Olli Matilainen
- & Carina I Holmberg
