Research Briefing |
Featured
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Article |
VIBRANT: spectral profiling for single-cell drug responses
Vibrational painting (VIBRANT) is a high-content single-cell phenotypic profiling method using mid-infrared imaging with vibrational probes for metabolic activity, which offers high accuracy with minimal batch effects to capture cellular responses to perturbation.
- Xinwen Liu
- , Lixue Shi
- & Wei Min
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Research Highlight |
Crosslinking mass spectrometry data bolster protein structure prediction
- Arunima Singh
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Analysis
| Open Access
Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins
An international blind study confirms that smFRET measurements on dynamic proteins are highly reproducible across instruments, analysis procedures and timescales, further highlighting the promise of smFRET for dynamic structural biology.
- Ganesh Agam
- , Christian Gebhardt
- & Thorben Cordes
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Editorial |
Single-cell proteomics: challenges and prospects
Single-cell proteomics is a challenging goal and an area of rapid methods development. This Focus issue highlights the many paths toward high-throughput, high-sensitivity measurements.
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Brief Communication |
Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy
A dual line-scanning approach increases imaging speed and reduces illumination levels in Brillouin microscopy, which is demonstrated on spheroids.
- Jitao Zhang
- , Milos Nikolic
- & Giuliano Scarcelli
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Method to Watch |
Annotating unknown metabolites
Computational approaches are pushing the limits of unknown metabolite annotation.
- Arunima Singh
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Method to Watch |
Fluorescence in structural biology
Advances in fluorescence microscopy and spectroscopy show their promise for applications that complement in situ structural biology methods like cryoelectron tomography.
- Rita Strack
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Research Highlight |
A closer look at FluoroCubes
Researchers explore the unique and puzzling photostability of DNA FluoroCubes. Moreover, they improve the probes’ performance and highlight their diverse applicability.
- Rita Strack
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Research Highlight |
Sensitive protein analysis with plexDIA
plexDIA increases throughput of proteomics analysis with low sample amounts.
- Arunima Singh
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Research Highlight |
Spatial proteomics with subcellular resolution
Deep Visual Proteomics combines the power of deep-learning-based image analysis with microdissection and ultrasensitive mass spectrometry to provide insights into the spatial proteome.
- Rita Strack
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Research Highlight |
Enhanced protein isoform characterization
Long-read proteogenomics helps delineate isoform diversity in full-length proteins.
- Arunima Singh
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Method to Watch |
Imaging without the labels
Advances in label-free microscopy are expanding experimental observations, from biophysics to cell biology and beyond.
- Rita Strack
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Method to Watch |
Deconstructing subcellular composition
High-throughput and ultrasensitive mass spectrometry–based approaches are gaining ground in subcellular compositional analysis.
- Arunima Singh
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Research Highlight |
Towards resolving proteomes in single cells
Development of methods for single-cell proteomics increases depth and throughput
- Arunima Singh
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Article |
ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling
ABEL-FRET combines anti-Brownian trapping for tether-free observation of biomolecules in solution with smFRET. ABEL-FRET offers ultrahigh resolution of FRET efficiency and enables simultaneous hydrodynamic profiling of molecular composition.
- Hugh Wilson
- & Quan Wang
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Research Highlight |
Probing dynamic proteomes
Global protein structure readouts help detect protein functional changes.
- Arunima Singh
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Method to Watch |
Glycoproteomics
Glycoproteomics is coming of age, thanks to advances in instrumentation, experimental methodologies and computational search algorithms.
- Arunima Singh
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Review Article |
Advances and challenges for fluorescence nanothermometry
This Review on nanothermometry introduces the many types of nanothermometers and their cellular and in vivo applications, as well as best practices for accurate measurements.
- Jiajia Zhou
- , Blanca del Rosal
- & Dayong Jin
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Brief Communication |
High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy
Stimulated Brillouin scattering microscopy overcomes the trade-off between acquisition speed and spectral resolution in spontaneous Brillouin scattering microscopy and allows visualization of elasticity and viscosity, as shown in C. elegans.
- Itay Remer
- , Roni Shaashoua
- & Alberto Bilenca
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Research Highlight |
Imaging single glycan molecules
Direct imaging of carbohydrates using electrospray ion beam deposition and scanning tunneling microscopy.
- Arunima Singh
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News & Views |
A new way to see RNAs
Producing reliable atomic- or close-to-atomic-resolution structures of RNA-only molecules has been a formidable task. Ribosolve can solve sub-nanometer-resolution cryo-EM structures of unbound RNA molecules with unprecedented accuracy and speed.
- Jane S. Richardson
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Article |
Mid-infrared metabolic imaging with vibrational probes
Small vibrational tags (azide, 13C-edited carbonyl and deuterium-labeled probes) were introduced as metabolic probes for mid-infrared imaging. The tags allow unprecedented in situ visualization of metabolism in cells and animals with high information throughput.
- Lixue Shi
- , Xinwen Liu
- & Wei Min
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Brief Communication |
Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography
Theoretical and experimental guidelines to determine laser intensity for single-photon excitation in microcrystals for ultrafast pump–probe experiments by serial femtosecond crystallography.
- Marie Luise Grünbein
- , Miriam Stricker
- & Ilme Schlichting
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Matters Arising |
Reply to: Spatial heterogeneity in molecular brightness
- Michael R. Stoneman
- , Gabriel Biener
- & Valerică Raicu
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Research Highlight |
Mass Spectrometry Search Tool (MASST)
Searching mass spectra against publicly available tandem MS databases just got easier.
- Arunima Singh
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Research Highlight |
Ligandable proteome mapping
Stereochemically defined chemical probes aid discovery of ligandable proteins.
- Arunima Singh
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Research Highlight |
Super-resolution Raman imaging
Higher-order processes can be harnessed for super-resolution CARS vibrational microscopy.
- Nina Vogt
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Review Article |
Brillouin microscopy: an emerging tool for mechanobiology
This Review discusses the principles, advantages and limitations of Brillouin microscopy, a non-invasive tool for measuring mechanical properties of biological samples in three dimensions.
- Robert Prevedel
- , Alba Diz-Muñoz
- & Giuseppe Antonacci
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Perspective |
Biological imaging of chemical bonds by stimulated Raman scattering microscopy
This Perspective summarizes advances in stimulated Raman spectroscopy (SRS) instruments and probes, and highlights biological discoveries made with these technologies.
- Fanghao Hu
- , Lixue Shi
- & Wei Min
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Brief Communication |
A general method to quantify ligand-driven oligomerization from fluorescence-based images
Fluorescence intensity fluctuation spectrometry provides a rapid and accurate measurement of the identity, abundance and stability of protein oligomers.
- Michael R. Stoneman
- , Gabriel Biener
- & Valerică Raicu
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Research Highlight |
Spatially resolved isotope labeling
Electron-energy-loss spectroscopy with a scanning transmission electron microscope enables spatially resolved imaging of isotope-labeled molecules.
- Christian Schnell
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Research Highlight |
RNA-binding proteome redux
Scientists exploit the basic physicochemical properties of protein–RNA complexes to paint a dynamic portrait of the RNA-binding proteome.
- Stéphane Larochelle
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Brief Communication |
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
A single-photon detector array enables robust and versatile image scanning microscopy (ISM) on any confocal microscope. This implementation makes super-resolution FLIM possible and eases a transition from confocal microscopy to ISM.
- Marco Castello
- , Giorgio Tortarolo
- & Giuseppe Vicidomini
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Research Highlight |
Membrane protein complexes exposed
A vesicle-based mass spectrometry method enables the analysis of protein complexes in native membranes.
- Karin Kuehnel
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Analysis
| Open Access
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
A multi-laboratory study finds that single-molecule FRET is a reproducible and reliable approach for determining accurate distances in dye-labeled DNA duplexes.
- Björn Hellenkamp
- , Sonja Schmid
- & Thorsten Hugel
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Correspondence |
Reply to ‘Water content, not stiffness, dominates Brillouin spectroscopy measurements in hydrated materials’
- Giuliano Scarcelli
- & Seok Hyun Yun
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Research Highlight |
Weighing single proteins with light
Interferometric scattering microscopy enables quantitative mass imaging and biophysical characterization of single biomolecules in solution.
- Rita Strack
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Research Highlights |
Tracking the protein–metabolite interactome
A method combining limited proteolysis with mass spectrometry systematically detects protein–metabolite interactions.
- Allison Doerr
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Article |
Supermultiplexed optical imaging and barcoding with engineered polyynes
A class of probes enables highly multiplexed imaging and barcoding applications with Raman microscopy.
- Fanghao Hu
- , Chen Zeng
- & Wei Min
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Research Highlights |
Sequencing for carbohydrates
A technique combining mass spectrometry and infrared spectroscopy shows promise for unambiguous sequencing of linear oligosaccharides.
- Allison Doerr
VIBRANT: a phenotyping method for drug discovery using vibrational spectroscopy