Featured
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RNA secondary structure packages evaluated and improved by high-throughput experiments
The EternaBench dataset of synthetic RNA constructs was used to directly compare RNA secondary structure prediction software packages on ensemble-oriented prediction tasks and used to train the EternaFold model for improved performance.
- Hannah K. Wayment-Steele
- , Wipapat Kladwang
- & Rhiju Das
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Directing cellular information flow via CRISPR signal conductors
The integration of ligand-responsive riboswitches with single guide RNAs allows Cas9–effector fusions to be targeted in response to ligands and thereby translate a cellular signal into a downstream readout of choice.
- Yuchen Liu
- , Yonghao Zhan
- & Zhiming Cai
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Reprogramming eukaryotic translation with ligand-responsive synthetic RNA switches
Ligand-triggered ribosomal frameshifting allows control of the relative stoichiometry of two proteins and enables the building of logic gates from a single mRNA.
- Andrew V Anzalone
- , Annie J Lin
- & Virginia W Cornish
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High-throughput cellular RNA device engineering
Screening for tertiary-interaction responsiveness in large RNA ribozyme-aptamer libraries to ligands identifies RNA devices with improved activation ratios and ligand sensitivities.
- Brent Townshend
- , Andrew B Kennedy
- & Christina D Smolke
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News & Views |
Expanding the synthetic ribonucleoprotein world in cells
Protein-responsive ribozymes generated by a streamlined design process will expand the plug-and-play toolbox for synthetic biologists.
- Kei Endo
- , Callum Parr
- & Hirohide Saito
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A general design strategy for protein-responsive riboswitches in mammalian cells
Designer ribozymes show protein-responsive translational control and can be combined with transcriptional control elements to program complex gene circuits.
- Simon Ausländer
- , Pascal Stücheli
- & Martin Fussenegger
Identifying the structures of individual RNA isoforms inside cells