Proteins articles within Polymer Journal

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• Original Article | 06 March 2013

  A novel approach for the parallel synthesis of glycopeptides by combining solid-phase peptide synthesis and dendrimer-supported enzymatic modifications

  Toward parallel enzymatic synthesis of biologically and pharmaceutically important glycopeptides, we investigated the feasibility of aminooxy-functionalized G7 PAMAM dendrimer derivative in the enzyme-assisted synthesis of glycopeptides having oligosaccharide side chains. The G7 PAMAM dendrimer was proved to become a nice supporting material that exhibited 99% recovery in a simple centrifugal ultrafiltration (UF) procedure in comparison with the hollow-fiber UF module (86%) used in the high-performance liquid chromatography-based automated glycan synthesizer. An optimization of the protocol was performed for the synthesis of glycopeptides bearing di-, tri- and tetrasaccharide branches.

  • Takahiko Matsushita
  • , Seiji Handa
  •  & Shin-Ichiro Nishimura

• Original Article | 18 August 2010

  Polysaccharide nanogel–cyclodextrin system as an artificial chaperone for in vitro protein synthesis of green fluorescent protein

  The molecular chaperone function of polysaccharide nanogel was demonstrated for the folding of newly synthesized green fluorescent protein (GFP) in a cell-free protein synthesis system. Nanogel comprising cholesteryl group-bearing pullulan (CHP) trapped unfolded or partially folded GFP expressed in the cell-free system. The structure of the CHP nanogel was disrupted by the addition of cyclodextrins, and the protein complexed with the nanogel was released and folded into the mature form.

  • Yoshihiro Sasaki
  • , Yuta Nomura
  •  & Kazunari Akiyoshi