Featured
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Article |
The evolution of lncRNA repertoires and expression patterns in tetrapods
Evolutionary study of long noncoding RNA (lncRNA) repertoires and expression patterns in 11 tetrapod species identifies approximately 11,000 primate-specific lncRNAs and 2,500 highly conserved lncRNAs, including approximately 400 genes that are likely to have ancient origins; many lncRNAs, particularly ancient ones, are actively regulated and may function mainly in embryonic development.
- Anamaria Necsulea
- , Magali Soumillon
- & Henrik Kaessmann
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Letter |
Genetics of single-cell protein abundance variation in large yeast populations
A new method for identifying genetic loci that influence protein expression in budding yeast reveals considerable complexity in how genetic variation shapes the proteome.
- Frank W. Albert
- , Sebastian Treusch
- & Leonid Kruglyak
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Letter |
A statin-dependent QTL for GATM expression is associated with statin-induced myopathy
Exploration of the interacting effect of statin exposure and genetic variation on gene expression identifies a cis-eQTL that is differentially associated with expression of the GATM gene, which encodes a rate-limiting enzyme involved in creatine synthesis, and that is associated with incidence of statin-induced myopathy, the major adverse effect of statin treatment.
- Lara M. Mangravite
- , Barbara E. Engelhardt
- & Ronald M. Krauss
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Research Highlights |
RNA tails time protein production
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News & Views |
Erase for a new start
Tet proteins regulate gene expression by removing methyl groups from DNA bases. This activity may be a facilitating step in turning on the cell-division pathway that produces sperm and egg cells. See Letter p.443
- Sylvain Guibert
- & Michael Weber
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Article |
Generation of functional thyroid from embryonic stem cells
Transient overexpression of the transcription factors NKX2-1 and PAX8 in a murine cell model is shown to direct the differentiation of embryonic stem cells towards a thyroid follicular cell lineage; the resulting three-dimensional thyroid follicles created by subsequent thyrotropin treatment show hallmarks of thyroid function in vitro and rescue thyroid function in vivo when transplanted into athyroid mice, adding to our understanding of the molecular mechanisms underlying thyroid development.
- Francesco Antonica
- , Dominika Figini Kasprzyk
- & Sabine Costagliola
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Brief Communications Arising |
Is irisin a human exercise gene?
- James A. Timmons
- , Keith Baar
- & Philip J. Atherton
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Letter |
APJ acts as a dual receptor in cardiac hypertrophy
APJ is shown to be a bifunctional receptor for both mechanical stretch and the endogenous peptide apelin, a finding that is important for the development of APJ agonists to treat heart failure.
- Maria Cecilia Scimia
- , Cecilia Hurtado
- & Pilar Ruiz-Lozano
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News & Views |
A sporadic super state
It seems that embryonic stem cells regularly pass through a transient state during which they can generate all the cell types of an animal, including those of the placenta. See Article p.57
- Azim Surani
- & Julia Tischler
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Letter |
A map of the cis-regulatory sequences in the mouse genome
A genomic map of nearly 300,000 potential cis-regulatory sequences determined from diverse mouse tissues and cell types reveals active promoters, enhancers and CCCTC-binding factor sites encompassing 11% of the mouse genome and significantly expands annotation of mammalian regulatory sequences.
- Yin Shen
- , Feng Yue
- & Bing Ren
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Research Highlights |
Cancer gene shifts chromatin
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News & Views |
Running to stand still
Transcription factors regulate the expression of genes by binding to certain DNA sequences. But the outcome can be markedly different, depending on whether the binding is stable or short-lived. See Letter p.251
- Tommy Kaplan
- & Nir Friedman
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News |
Monkey genetics track social status
Low-ranking animals have overactive immune genes that may lead to ill health.
- Zoë Corbyn
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Letter |
Deregulated MYC expression induces dependence upon AMPK-related kinase 5
Oncogenic levels of MYC, the deregulation of which is implicated in many human tumours, are shown to establish a dependence on ARK5, offering insights into potential therapeutic strategies.
- Lidan Liu
- , Jannes Ulbrich
- & Daniel J. Murphy
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Letter |
Deleted in colorectal carcinoma suppresses metastasis in p53-deficient mammary tumours
In a mouse model of mammary carcinoma, loss of deleted in colorectal cancer (DCC) promotes metastasis formation, and in cell cultures derived from p53-deficient mouse mammary tumours DCC expression controls netrin-1-dependent cell survival, supporting the function of DCC as a context-dependent tumour suppressor that limits survival of disseminated tumour cells.
- Paul Krimpenfort
- , Ji-Ying Song
- & Anton Berns
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Article |
Piezo proteins are pore-forming subunits of mechanically activated channels
Large transmembrane proteins of the Piezo family assemble as tetramers to form a new class of ion channel that can be activated by mechanical force.
- Bertrand Coste
- , Bailong Xiao
- & Ardem Patapoutian
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Letter |
Control of ground-state pluripotency by allelic regulation of Nanog
Tight regulation of Nanog dose at the chromosome level is important for the acquisition of pluripotency during development.
- Yusuke Miyanari
- & Maria-Elena Torres-Padilla
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Letter |
Expression of tumour-specific antigens underlies cancer immunoediting
This paper illustrates that immunosurveillance and immunoediting can occur in an oncogene-driven endogenous tumour model provided that the tumours carry strong neoantigens not present in the host.
- Michel DuPage
- , Claire Mazumdar
- & Tyler Jacks
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Letter |
Enhancer decommissioning by LSD1 during embryonic stem cell differentiation
In embryonic stem cells, the histone demethylase LSD1 occupies the enhancers of active genes and, together with the NuRD complex, decommissions the enhancers during differentiation.
- Warren A. Whyte
- , Steve Bilodeau
- & Richard A. Young
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Article |
Endothelial and perivascular cells maintain haematopoietic stem cells
The cellular sources of stem cell factor, a major haematopoietic stem cell (HSC) niche cytokine required for HSC maintenance, are identified.
- Lei Ding
- , Thomas L. Saunders
- & Sean J. Morrison
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Article |
A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis
In mice, expression of PGC1-α in muscles is shown to stimulate expression of FNDC5, which is cleaved and secreted in the circulation as the newly identified hormone irisin; on exercise, this hormone stimulates browning of subcutaneous adipose tissue.
- Pontus Boström
- , Jun Wu
- & Bruce M. Spiegelman
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Letter |
Cysteinyl leukotriene type I receptor desensitization sustains Ca2+-dependent gene expression
Rather than turning off the biological response, desensitization of the cysteinyl leukotriene type I receptor sustains long-term signalling in the immune system.
- Siaw-Wei Ng
- , Daniel Bakowski
- & Anant B. Parekh
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Research Highlights |
How the brain became human
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Obituary |
Har Gobind Khorana (1922–2011)
Chemical biologist who helped to establish the genetic code.
- Uttam L. RajBhandary
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Research Highlights |
Gene signature links to drug target
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Article |
The evolution of gene expression levels in mammalian organs
- David Brawand
- , Magali Soumillon
- & Henrik Kaessmann
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Letter |
Absence of effects of Sir2 overexpression on lifespan in C. elegans and Drosophila
- Camilla Burnett
- , Sara Valentini
- & David Gems
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Article |
lincRNAs act in the circuitry controlling pluripotency and differentiation
- Mitchell Guttman
- , Julie Donaghey
- & Eric S. Lander
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Article |
Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling
- Wim de Lau
- , Nick Barker
- & Hans Clevers
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Letter |
A role for glia in the progression of Rett’s syndrome
- Daniel T. Lioy
- , Saurabh K. Garg
- & Gail Mandel
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News & Views |
The autism disconnect
Separating primary from secondary changes in the autistic brain has long been a research goal. With knowledge of wide-ranging molecular deficits, identification of the best therapeutic targets becomes a priority. See Letter p.380
- Željka Korade
- & Károly Mirnics
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Article |
Global quantification of mammalian gene expression control
- Björn Schwanhäusser
- , Dorothea Busse
- & Matthias Selbach
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Letter |
Neuronal activity is required for the development of specific cortical interneuron subtypes
- Natalia V. De Marco García
- , Theofanis Karayannis
- & Gord Fishell
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Research Highlights |
Molecular biology: Long RNAs turn up gene expression
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News & Views |
The coherent Mediator
Enhancer sequences increase gene transcription with the help of a co-activator complex, the Mediator. Another protein complex — cohesin — seems to work with Mediator to bring together enhancers and promoters. See Article p. 430
- Rolf Ohlsson
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Brief Communications Arising |
Can controversies be put to REST?
- Helle F. Jørgensen
- & Amanda G. Fisher
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Letter |
IκBβ acts to inhibit and activate gene expression during the inflammatory response
Nuclear hypophosphorylated IκBβ is shown to bind p65:c-Rel dimers and maintain prolonged expression of TNF-α in response to stimulation by lipopolysaccharide.
- Ping Rao
- , Mathew S. Hayden
- & Sankar Ghosh
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Article |
Mediator and cohesin connect gene expression and chromatin architecture
Gene activation may involve the formation of a DNA loop that connects enhancer-bound transcription factors with the transcription apparatus at the core promoter. But this process is not well understood. Here, two proteins, mediator and cohesin, are shown to connect the enhancers and core promoters of active genes in embryonic stem cells. These proteins seem to generate cell-type-specific DNA loops linked to the gene expression program of each cell.
- Michael H. Kagey
- , Jamie J. Newman
- & Richard A. Young
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Research Highlights |
Behaviour: Brain-changing battles
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Letter |
IκBζ regulates TH17 development by cooperating with ROR nuclear receptors
Interleukin-17-producing helper T (TH17) cells are a distinct T-cell subset characterized by its role in autoimmune disease. Here it is shown that the development of TH17 cells requires the transcription factor IκBζ, as well as nuclear receptors of the ROR family. Mice lacking IκBζ have a defect in TH17 development and are resistant to the induction of experimental autoimmune encephalomyelitis. The study points to some new potential molecular targets for drugs to treat autoimmune disease.
- Kazuo Okamoto
- , Yoshiko Iwai
- & Hiroshi Takayanagi
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Article |
Zscan4 regulates telomere elongation and genomic stability in ES cells
Zscan4 is shown to be involved in maintaining telomeres in embryonic stem (ES) cells. Only 5% of ES cells express Zscan4 at a given time, but nearly all ES cells activate Zscan4 at least once within nine passages. The transient Zscan4-positive state is associated with rapid telomere extension by telomere recombination and upregulation of meiosis–specific homologous recombination genes. Knocking down Zscan4 shortens telomeres, increases karyotype abnormalities and spontaneous sister chromatid exchange, and slows down cell proliferation until reaching crisis by eight passages.
- Michal Zalzman
- , Geppino Falco
- & Minoru S. H. Ko
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Research Highlights |
Metabolism: Warm milk
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Letter |
Understanding mechanisms underlying human gene expression variation with RNA sequencing
There is much interest in understanding the genetic mechanisms that underlie individual variations in gene expression. Here, RNA sequencing has been used to study gene expression in lymphoblastoid cell lines derived from Nigerian individuals for whom extensive genotype information is known. Numerous genetic determinants of variation in gene expression were identified, including variation in transcription, splicing and allele-specific expression.
- Joseph K. Pickrell
- , John C. Marioni
- & Jonathan K. Pritchard
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Letter |
Transcriptome genetics using second generation sequencing in a Caucasian population
Here, sequencing has been used to characterize the mRNA fraction of the transcriptome in Caucasian individuals, to provide a fine-scale view of transcriptomes and to identify genetic variants that affect alternative splicing. Measuring allele-specific expression identified rare expression quantitative trait loci (eQTLs) and allelic differences in transcript structure, revealing new properties of genetic effects on the transcriptome.
- Stephen B. Montgomery
- , Micha Sammeth
- & Emmanouil T. Dermitzakis
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Letter |
Transcriptional control of preadipocyte determination by Zfp423
An understanding of how fat cells (adipocytes) develop will contribute to our understanding of obesity. The differentiation of committed preadipocytes into adipocytes is known to be controlled by PPARγ and several other transcription factors. But what turns a cell into a preadipocyte? Here, the zinc-finger protein Zfp423 is identified as a transcriptional regulator of preadipocyte determination.
- Rana K. Gupta
- , Zoltan Arany
- & Bruce M. Spiegelman
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Letter |
Rere controls retinoic acid signalling and somite bilateral symmetry
The vertebrate body plan shows marked bilateral symmetry, although this can be disrupted in conditions such as scoliosis. Here, a mutation in Rere is found that leads to the formation of asymmetrical somites in mouse embryos; furthermore, Rere is shown to control retinoic acid signalling, which is required to maintain somite symmetry by interacting with Fgf8. The results provide insight into how bilateral symmetry is maintained.
- Gonçalo C. Vilhais-Neto
- , Mitsuji Maruhashi
- & Olivier Pourquié
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Article |
Variability in gene expression underlies incomplete penetrance
Even genetically identical organisms in homogeneous environments vary, indicating that randomness in developmental processes such as gene expression may generate phenotypic diversity. Intestinal specification in the nematode Caenorhabditis elegans, in which wild-type cell fate is invariant and controlled by a small transcriptional network, is now studied. The results demonstrate that mutations in developmental networks can expose stochastic variability in gene expression, leading to phenotypic variation.
- Arjun Raj
- , Scott A. Rifkin
- & Alexander van Oudenaarden