Featured
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| Open AccessNumerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9
- Justin A. Jarrell
- , Brandon J. Sytsma
- & Ryan S. Pawell
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| Open AccessAn efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
- Jens Hamar
- & Dietmar Kültz
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| Open AccessDevelopment of a pVEC peptide-based ribonucleoprotein (RNP) delivery system for genome editing using CRISPR/Cas9 in Chlamydomonas reinhardtii
- Seongsu Kang
- , Seungjib Jeon
- & Yeu-Chun Kim
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| Open AccessA comparison of AAV-vector production methods for gene therapy and preclinical assessment
- Marcus Davidsson
- , Matilde Negrini
- & Andreas Heuer
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| Open AccessBaculovirus-free insect cell expression system for high yield antibody and antigen production
- Janin Korn
- , Dorina Schäckermann
- & Maren Schubert
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| Open AccessImpedance analysis of adherent cells after in situ electroporation-mediated delivery of bioactive proteins, DNA and nanoparticles in µL-volumes
- Judith A. Stolwijk
- & Joachim Wegener
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| Open AccessGeneration of a Matrix Gla (Mgp) floxed mouse, followed by conditional knockout, uncovers a new Mgp function in the eye
- Teresa Borrás
- , Dale O. Cowley
- & Kumar Pandya
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| Open AccessHigh-throughput continuous-flow microfluidic electroporation of mRNA into primary human T cells for applications in cellular therapy manufacturing
- Charles A. Lissandrello
- , Jose A. Santos
- & Vishal Tandon
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| Open AccessOptimization of human papillomavirus-based pseudovirus techniques for efficient gene transfer
- Timra D. Gilson
- , Ryan T. Gibson
- & Elliot J. Androphy
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| Open AccessViromers as carriers for mRNA-mediated expression of therapeutic molecules under inflammatory conditions
- Edith Jansig
- , Stefanie Geissler
- & Holger Cynis
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| Open AccessA highly efficient non-viral process for programming mesenchymal stem cells for gene directed enzyme prodrug cancer therapy
- Yoon Khei Ho
- , Jun Yung Woo
- & Heng-Phon Too
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| Open AccessA double-Flp-in method for stable overexpression of two genes
- Ole Jensen
- , Salim Ansari
- & Jürgen Brockmöller
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Article
| Open AccessCRISPR/Cas12a mediated knock-in of the Polled Celtic variant to produce a polled genotype in dairy cattle
- Felix Schuster
- , Patrick Aldag
- & Björn Petersen
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Article
| Open AccessDNA unchained: two assays to discover and study inhibitors of the DNA clustering function of barrier-to-autointegration factor
- Michael Burger
- , Caroline Schmitt-Koopmann
- & Jean-Christophe Leroux
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Article
| Open AccessInduction of a local muscular dystrophy using electroporation in vivo: an easy tool for screening therapeutics
- Aline Derenne
- , Alexandra Tassin
- & Alexandre Legrand
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Article
| Open AccessAAV8 Ins1-Cre can produce efficient β-cell recombination but requires consideration of off-target effects
- Adam Ramzy
- , Eva Tudurí
- & Timothy J. Kieffer
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Article
| Open AccessAdeno-associated virus-binding antibodies detected in cats living in the Northeastern United States lack neutralizing activity
- Kei Adachi
- , Gregory A. Dissen
- & Hiroyuki Nakai
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Article
| Open AccessAbrogation of PRRSV infectivity by CRISPR-Cas13b-mediated viral RNA cleavage in mammalian cells
- Jin Cui
- , Navapon Techakriengkrai
- & Sanipa Suradhat
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Article
| Open AccessLipid Nanoparticle Formulation Increases Efficiency of DNA-Vectored Vaccines/Immunoprophylaxis in Animals Including Transchromosomic Bovines
- Eric M. Mucker
- , Priya P. Karmali
- & Jay W. Hooper
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| Open AccessMicrofluidic Electroporation Coupling Pulses of Nanoseconds and Milliseconds to Facilitate Rapid Uptake and Enhanced Expression of DNA in Cell Therapy
- An-Yi Chang
- , Xuan Liu
- & Shengnian Wang
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| Open AccessIn vivo biodistribution analysis of transmission competent and defective RNA virus-based episomal vector
- Yumiko Komatsu
- , Chiaki Tanaka
- & Keizo Tomonaga
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Article
| Open AccessIn vivo engineering of lymphocytes after systemic exosome-associated AAV delivery
- Cort B. Breuer
- , Killian S. Hanlon
- & Casey A. Maguire
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| Open AccessmRNA Transfection-Induced Activation of Primary Human Monocytes and Macrophages: Dependence on Carrier System and Nucleotide Modification
- Hanieh Moradian
- , Toralf Roch
- & Manfred Gossen
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| Open AccessThe effects of electroporation buffer composition on cell viability and electro-transfection efficiency
- Joseph J. Sherba
- , Stephen Hogquist
- & Jeffrey D. Zahn
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Article
| Open AccessDevelopment of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach
- Shengli Xu
- , Enhui Lai
- & Beibei Zheng
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Article
| Open AccessLyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum
- Gokulapriya Govindarajalu
- , Zeba Rizvi
- & Puran Singh Sijwali
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| Open AccessEffective lung-targeted RNAi in mice with peptide-based delivery of nucleic acid
- Kaido Kurrikoff
- , Krista Freimann
- & Ülo Langel
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| Open AccessEpisomal vectors based on S/MAR and the β-globin Replicator, encoding a synthetic transcriptional activator, mediate efficient γ-globin activation in haematopoietic cells
- Eleana F. Stavrou
- , Emannuouil Simantirakis
- & Aglaia Athanassiadou
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| Open AccessPrevention of quality decline and delivery of siRNA using exogenous TCTP translocation across the zona pellucida in mouse oocytes
- Hyuk-Joon Jeon
- , Guang-Yu Bai
- & Jeong Su Oh
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Article
| Open AccessCell-based interferon gene therapy using proliferation-controllable, interferon-releasing mesenchymal stem cells
- Mari Tsujimura
- , Kosuke Kusamori
- & Makiya Nishikawa
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Article
| Open AccessAdeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly
- Dinh To Le
- , Marco T. Radukic
- & Kristian M. Müller
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Article
| Open AccessProduction of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells
- Ondřej Vaněk
- , Petra Celadova
- & James R. Carlyle
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Article
| Open AccessIn vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
- Robin M. H. Rumney
- , Stuart A. Lanham
- & Richard O. C. Oreffo
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Article
| Open AccessSyncytin 1 dependent horizontal transfer of marker genes from retrovirally transduced cells
- Berna Uygur
- , Kamran Melikov
- & Leonid V. Chernomordik
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Article
| Open AccessEnzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier
- Taufiq Indra Rukmana
- , Gabriela Moran
- & Yoichiroh Hosokawa
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Article
| Open AccessHigher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
- Alicia Gamboa
- , Selina F. Urfano
- & Katarzyna Slowinska
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| Open AccessHighly efficient ex vivo lentiviral transduction of primary human pancreatic exocrine cells
- Jeetindra R. A. Balak
- , Natascha de Graaf
- & Françoise Carlotti
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| Open AccessA Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular Therapies
- Nathan Moore
- , John R. Chevillet
- & Kenneth Kotz
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| Open AccessNon-viral vector based gene transfection with human induced pluripotent stem cells derived cardiomyocytes
- Shihua Tan
- , Zhonghao Tao
- & Lei Ye
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| Open AccessProduction of adeno-associated virus vectors for in vitro and in vivo applications
- Toyokazu Kimura
- , Beatriz Ferran
- & Markus Michael Bachschmid
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| Open AccessMessenger RNA translation enhancement by immune evasion proteins: a comparative study between EKB (vaccinia virus) and NS1 (influenza A virus)
- Yi Liu
- , Jas Min Chin
- & Kyle K. L. Phua
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| Open AccessSleeping Beauty Transposon-Mediated Asparaginase Gene Delivery by a Nanoparticle Platform
- Jen-Hsuan Chang
- , Kurt Yun Mou
- & Chung-Yuan Mou
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| Open AccessConsiderations related to the use of short neuropeptide promoters in viral vectors targeting hypothalamic neurons
- N. Kakava-Georgiadou
- , C. Bullich-Vilarrubias
- & R. A. H. Adan
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Article
| Open AccessAAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells
- Eva Tudurí
- , Maria M. Glavas
- & Timothy J. Kieffer
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Article
| Open AccessSimplified platform for mosaic in vivo analysis of cellular maturation in the developing heart
- Julie Goudy
- , Trevor Henley
- & Michael Bressan
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| Open AccessThe adeno-associated virus rh10 vector is an effective gene transfer system for chronic spinal cord injury
- Yutaka Hoshino
- , Kenji Nishide
- & Hideyuki Okano
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| Open AccessDiphtheria Toxin A-Resistant Cell Lines Enable Robust Production and Evaluation of DTA-Encoding Lentiviruses
- Margaret J. Lange
- , Terri D. Lyddon
- & Marc C. Johnson
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| Open AccessBile-duct proliferation as an unexpected side-effect after AAV2-LDLR gene transfer to rabbit liver
- Elisa Hytönen
- , Anniina Laurema
- & Seppo Ylä-Herttuala
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| Open AccessA note on retrograde gene transfer efficiency and inflammatory response of lentiviral vectors pseudotyped with FuG-E vs. FuG-B2 glycoproteins
- Soshi Tanabe
- , Shiori Uezono
- & Masahiko Takada