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Cellular transfection using rapid decrease in hydrostatic pressure
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A multicellular brain spheroid model for studying the mechanisms and bioeffects of ultrasound-enhanced drug penetration beyond the blood‒brain barrier
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Pan msr gene deleted strain of Salmonella Typhimurium suffers oxidative stress, depicts macromolecular damage and attenuated virulence
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Expression of a large coding sequence: Gene therapy vectors for Ataxia Telangiectasia
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Subcellular distribution of the rAAV genome depends on genome structure
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A novel sorting signal for RNA packaging into small extracellular vesicles
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2-Photon imaging of fluorescent proteins in living swine
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Moonlighting genes harbor antisense ORFs that encode potential membrane proteins
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Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth
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The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments
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Scalable continuous-flow electroporation platform enabling T cell transfection for cellular therapy manufacturing
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High spontaneous integration rates of end-modified linear DNAs upon mammalian cell transfection
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Full-length human dystrophin on human artificial chromosome compensates for mouse dystrophin deficiency in a Duchenne muscular dystrophy mouse model
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Targeting DNA repair pathways with B02 and Nocodazole small molecules to improve CRIS-PITCh mediated cassette integration in CHO-K1 cells
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Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery
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Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice
- Manabu Ozawa
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Effects of pre-existing anti-adenovirus antibodies on transgene expression levels and therapeutic efficacies of arming oncolytic adenovirus
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Development of a novel electroporation method for the oyster parasite Perkinsus marinus
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Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import
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An efficient rAAV vector for protein expression in cortical parvalbumin expressing interneurons
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Immediate protein expression from exogenous mRNAs in embryonic brain
- Masae Naruse
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Genome-wide identification and development of miniature inverted-repeat transposable elements and intron length polymorphic markers in tea plant (Camellia sinensis)
- Megha Rohilla
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Engineered DNA-encoded monoclonal antibodies targeting Plasmodium falciparum circumsporozoite protein confer single dose protection in a murine malaria challenge model
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Erythroid lineage-specific lentiviral RNAi vectors suitable for molecular functional studies and therapeutic applications
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A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide
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High rates of plasmid cotransformation in E. coli overturn the clonality myth and reveal colony development
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Muscle regeneration affects Adeno Associated Virus 1 mediated transgene transcription
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Improved in-vivo airway gene transfer via magnetic-guidance, with protocol development informed by synchrotron imaging
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Evaluation of the α-casein (CSN1S1) locus as a potential target for a site-specific transgene integration
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AAV9-mediated functional screening for cardioprotective cytokines in Coxsackievirus-B3-induced myocarditis
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Stent-based delivery of AAV2 vectors encoding oxidation-resistant apoA1
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A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila overexpression transgenes
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Transiently expressed CRISPR/Cas9 induces wild-type dystrophin in vitro in DMD patient myoblasts carrying duplications
- Veronica Pini
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Transposase-CRISPR mediated targeted integration (TransCRISTI) in the human genome
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Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression
- Anna V. Starikova
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Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors
- Jaime Lopera-Madrid
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Microfluidic transfection of mRNA into human primary lymphocytes and hematopoietic stem and progenitor cells using ultra-fast physical deformations
- Jocelyn Loo
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Construction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice
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Effect of AAV-mediated overexpression of ATF5 and downstream targets of an integrated stress response in murine skeletal muscle
- Madelaine C. Brearley-Sholto
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Concentration of Na+-taurocholate-cotransporting polypeptide expressed after in vitro-transcribed mRNA transfection determines susceptibility of hepatoma cells for hepatitis B virus
- Andreas Oswald
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A guide in lentiviral vector production for hard-to-transfect cells, using cardiac-derived c-kit expressing cells as a model system
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Aluminum particles generated during millisecond electric pulse application enhance adenovirus-mediated gene transfer in L929 cells
- Angela Tesse
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Bovine endometrium-derived cultured cells are suitable for lipofection
- Mai Shiokawa
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Lentiviral and AAV-mediated expression of palivizumab offer protection against Respiratory Syncytial Virus infection
- Agata Antepowicz
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A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
- Frederic Strobl
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In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes
- M. Maschietto
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- & S. Vassanelli
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Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer
- Kazunori Watanabe
- , Tomoko Nawachi
- & Takashi Ohtsuki
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GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS
- Natsuko Ohashi
- , Tomoya Terashima
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HIV-1 sequences in lentiviral vector genomes can be substantially reduced without compromising transduction efficiency
- Helin Sertkaya
- , Mattia Ficarelli
- & Chad M. Swanson
Gene targeting in adult organs using in vivo cleavable donor plasmids for CRISPR-Cas9 and CRISPR-Cas12a