Double-strand DNA breaks

Definition

A double-strand DNA break (DSB) occurs or arises when both strands of the DNA duplex are severed, often as the result of ionizing radiation. They are particularly deleterious as the DNA ends can promote potentially lethal chromosomal rearrangements, and can be repaired by homologous recombination (HR) or non-homologous-end joining (NHEJ).

Latest Research and Reviews

  • Research | | open

    The choice between non-homologous end-joining and homologous recombination to repair a DNA double-strand break depends on activation of the end resection machinery. Here the authors show that SUMO E3 ligase CBX4 sumoylates subpopulation of CtIP to regulate recruitment to breaks and resection.

    • Isabel Soria-Bretones
    • , Cristina Cepeda-García
    • , Cintia Checa-Rodriguez
    • , Vincent Heyer
    • , Bernardo Reina-San-Martin
    • , Evi Soutoglou
    •  & Pablo Huertas
  • Research |

    Structure determination and functional analyses of budding yeast Rif1 reveal a novel, hooked N-terminal DNA-binding domain required for telomere maintenance and checkpoint control and show that Rif1's role in DNA-repair pathway choice is conserved in yeast and mammalian cells.

    • Stefano Mattarocci
    • , Julia K Reinert
    • , Richard D Bunker
    • , Gabriele A Fontana
    • , Tianlai Shi
    • , Dominique Klein
    • , Simone Cavadini
    • , Mahamadou Faty
    • , Maksym Shyian
    • , Lukas Hafner
    • , David Shore
    • , Nicolas H Thomä
    •  & Ulrich Rass
  • Research | | open

    The choice between homologous recombination and non-homologous end-joining is largely influenced by cell cycle. Here the authors show that APCCdh1 promotes homologous recombination by removing USP1, allowing polyubiquitinated histones to recruit BRCA1.

    • Kyungsoo Ha
    • , Chengxian Ma
    • , Han Lin
    • , Lichun Tang
    • , Zhusheng Lian
    • , Fang Zhao
    • , Ju-Mei Li
    • , Bei Zhen
    • , Huadong Pei
    • , Suxia Han
    • , Marcos Malumbres
    • , Jianping Jin
    • , Huan Chen
    • , Yongxiang Zhao
    • , Qing Zhu
    •  & Pumin Zhang
  • Reviews |

    In mammalian cells, DNA double-strand breaks (DSBs) are repaired predominantly by the non-homologous end joining (NHEJ) pathway, which includes subpathways that can repair different DNA-end configurations. Furthermore, the repair of some DNA-end configurations can be shunted to the auxiliary pathways of alternative end joining (a-EJ) or single-strand annealing (SSA).

    • Howard H. Y. Chang
    • , Nicholas R. Pannunzio
    • , Noritaka Adachi
    •  & Michael R. Lieber
  • Research | | open

    RAP80 helps to recruit BRCA1 to double-strand breaks, facilitating DNA damage responses. Here the authors report that phosphorylated USP13 deubiquitinates RAP80 after DNA damage, prompting recruitment to the break site.

    • Yunhui Li
    • , Kuntian Luo
    • , Yujiao Yin
    • , Chenming Wu
    • , Min Deng
    • , Lei Li
    • , Yuping Chen
    • , Somaira Nowsheen
    • , Zhenkun Lou
    •  & Jian Yuan
  • Research | | open

    DNA double strand breaks (DSBs) are among the most deleterious types of damage and there is strong evidence indicating a relationship between breaks and transcription. Here the authors provide a high-resolution, genome-wide map of induced DSBs and observe ATM-dependent transcriptional repression.

    • Fabio Iannelli
    • , Alessandro Galbiati
    • , Ilaria Capozzo
    • , Quan Nguyen
    • , Brian Magnuson
    • , Flavia Michelini
    • , Giuseppina D’Alessandro
    • , Matteo Cabrini
    • , Marco Roncador
    • , Sofia Francia
    • , Nicola Crosetto
    • , Mats Ljungman
    • , Piero Carninci
    •  & Fabrizio d’Adda di Fagagna

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