Article
|
Open Access
Featured
-
-
This Month |
Languages in the lab
Members of a lab often have a varied language background. This rich language diversity leads to lab dynamics that take mindful handling.
- Vivien Marx
-
Research Highlight |
Profiling double-strand break repair
Repair-seq enables efficient profiling of genetic factors involved in DNA double-strand break repair.
- Lin Tang
-
-
Research Highlight |
Surprising CRISPR roadblocks
Cas9 induces larger-than-anticipated mutations in mouse and human cells. In the latter, efficient editing depends on inhibition of the DNA-damage-repair protein p53.
- Nicole Rusk
-
-
Article |
Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics
A rapidly inducible, autoinhibited SpCas9 and quantitative assessment of double-strand cleavage and indel formation allow insights into Cas9 kinetics in cell lines.
- John C Rose
- , Jason J Stephany
- & Douglas M Fowler
-
Research Highlights |
DNA variants or DNA damage?
Cryptic, widespread DNA damage is commonly interpreted as sequence variation.
- Tal Nawy
-
News & Views |
CRISPR-Cas9-AID base editor is a powerful gain-of-function screening tool
Combining CRISPR-Cas9 with an enzyme that induces somatic hypermutations allows the rapid generation of diverse variants for gain-of-function screens.
- Cem Kuscu
- & Mazhar Adli
-
This Month |
Shankar Balasubramanian
A chemist intrigued by DNA and RNA structure and two-wheeled mountainous ascents.
- Vivien Marx
-
Brief Communication |
DSBCapture: in situ capture and sequencing of DNA breaks
Double-strand DNA breaks capture (DSBCapture) identifies in situ DSBs via the ligation of an Illumina adaptor into the break site and shows no bias for chromatin state or base composition. A genome-wide DSB profile shows breaks occurring more frequently in euchromatin and transcriptionally active regions.
- Stefanie V Lensing
- , Giovanni Marsico
- & Shankar Balasubramanian
-
Research Highlights |
Cas9 and the importance of asymmetry
Short single-stranded DNA donors that asymmetrically span the Cas9 cut site show high efficiency in homology-directed editing.
- Nicole Rusk
-
Methods in Brief |
Timed CRISPR action enhances homology-driven repair
-
Article |
Ribose-seq: global mapping of ribonucleotides embedded in genomic DNA
Ribose-seq allows ribose nucleotide (rNMP) incorporation to be detected genome-wide in DNA at single-base resolution and is demonstrated on budding yeast.
- Kyung Duk Koh
- , Sathya Balachander
- & Francesca Storici
-
Article |
Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing
Biotinylated tags bind double-strand breaks in genomic DNA; after enrichment and sequencing, this allows precise, genome-wide mapping of the breaks.
- Nicola Crosetto
- , Abhishek Mitra
- & Ivan Dikic
-
Brief Communication |
TALEN-mediated precise genome modification by homologous recombination in zebrafish
Gene targeting via homologous recombination is achieved in the zebrafish with TALENs and double-stranded DNA donors, expanding the range of experimental possibilities in this organism.
- Yao Zu
- , Xiangjun Tong
- & Shuo Lin
-
Brief Communication |
Coupling endonucleases with DNA end–processing enzymes to drive gene disruption
Coexpression of DNA end–processing enzymes with targeted nucleases improves the efficiency of gene disruption in mammalian cells.
- Michael T Certo
- , Kamila S Gwiazda
- & Andrew M Scharenberg
-
Tools in Brief |
Timing DNA repair
-
Article |
A scalable pipeline for highly effective genetic modification of a malaria parasite
Reported is a recombineering pipeline for efficient and large-scale gene modification in the mouse malarial parasite Plasmodium berghei.
- Claudia Pfander
- , Burcu Anar
- & Oliver Billker
-
News & Views |
Seeing the light: integrating genome engineering with double-strand break repair
The two-color traffic light reporter reads out what pathway is used to repair a DNA break and will increase insights into genome engineering.
- Matthew Porteus
-
Article |
Tracking genome engineering outcome at individual DNA breakpoints
A fluorescent reporter, named traffic light, reads out whether repair of a DNA break occurs by nonhomologous end-joining or by homologous recombination. It should enable the identification of factors that affect repair pathway choice and thus improved approaches for genome engineering.
- Michael T Certo
- , Byoung Y Ryu
- & Andrew M Scharenberg
-
Brief Communication |
Targeted gene deletions in C. elegans using transposon excision
Targeted deletion of genes within 25 kb of Mos1 transposons in the C. elegans genome is demonstrated. This should enable single-gene deletions of more than 8,000 genes for which deletions are currently not available in this organism.
- Christian Frøkjær-Jensen
- , M Wayne Davis
- & Erik M Jorgensen
Efficient high-precision homology-directed repair-dependent genome editing by HDRobust