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CRISPR-Cas9-AID base editor is a powerful gain-of-function screening tool

Nature Methods volume 13, pages 983–984 (2016)Cite this article

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Combining CRISPR-Cas9 with an enzyme that induces somatic hypermutations allows the rapid generation of diverse variants for gain-of-function screens.

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Figure 1: Schematics of CRISPR-AID-mediated somatic hypermutations.

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Authors and Affiliations

  1. Cem Kuscu and Mazhar Adli are in the Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA.,

    Cem Kuscu & Mazhar Adli

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  1. Cem Kuscu
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  2. Mazhar Adli
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Correspondence to Mazhar Adli.

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Kuscu, C., Adli, M. CRISPR-Cas9-AID base editor is a powerful gain-of-function screening tool. Nat Methods 13, 983–984 (2016). https://doi.org/10.1038/nmeth.4076

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