SecY and Sec61 translocons mediate the orderly insertion of transmembrane segments into the lipid bilayer during membrane-protein biogenesis. Reporting in this issue, Ismail et al. now use a SecM-based molecular force sensor to show that the translocon exerts a pulling force on the nascent chain that is capable of mechanical action at two distinct stages of the insertion process.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Rapoport, T.A. Nature 450, 663–669 (2007).
Ismail, N., Hedman, R., Schiller, N. & von Heijne, G. Nat. Struct. Mol. Biol. 19, 1018–1022 (2012).
Heinrich, S.U., Mothes, W., Brunner, J. & Rapoport, T. Cell 102, 233–244 (2000).
Martoglio, B., Hofmann, M., Brunner, J. & Dobberstein, B. Cell 81, 207–214 (1995).
Do, H., Falcone, D., Lin, J., Andrews, D. & Johnson, A. Cell 85, 369–378 (1996).
Pitonzo, D., Yang, Z., Matsumura, Y., Johnson, A. & Skach, W. Mol. Biol. Cell 20, 685–698 (2009).
McCormick, P.J., Miao, Y., Shao, Y., Lin, J. & Johnson, A. Mol. Cell 12, 329–341 (2003).
Meacock, S.L., Lecomte, F., Crawshaw, S. & High, S. Mol. Biol. Cell 13, 4114–4129 (2002).
Sadlish, H., Pitonzo, D., Johnson, A. & Skach, W. Nat. Struct. Mol. Biol. 12, 870–878 (2005).
Butkus, M.E., Prundeanu, L.B. & Oliver, D.B. J. Bacteriol. 185, 6719–6722 (2003).
Crowley, K.S., Liao, S., Worrell, V., Reinhart, G. & Johnson, A. Cell 78, 461–471 (1994).
Junne, T., Kicik, L. & Spiess, M. Mol. Biol. Cell 21, 1662–1670 (2010).
Devaraneni, P.K. et al. Cell 146, 134–147 (2011).
Kida, Y., Morimoto, F. & Sakaguchi, M.J. J. Biol. Chem. 284, 2861–2866 (2009).
Lu, Y. et al. J. Biol. Chem. 273, 568–576 (1998).
Higy, M., Junne, T. & Spiess, M. Biochemistry 43, 12716–12722 (2004).
Hessa, T. et al. Nature 450, 1026–1030 (2007).
Skach, W.R. Nat. Struct. Mol. Biol. 16, 606–612 (2009).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Kim, S., Skach, W. Two-step insertion at the SecY translocon. Nat Struct Mol Biol 19, 975–977 (2012). https://doi.org/10.1038/nsmb.2402
Published:
Issue Date:
DOI: https://doi.org/10.1038/nsmb.2402
